基于纸张的光测定用于血型和抗体标位的确定,使用长期存储在环境中的生物工程RBC
Yuxuan Li1,2, Yulong Zhang1, Shengqiang Liang3
1Academy of Military Medical Sciences, Beijing, China.
Nature communications
|February 5, 2026
概括
这项研究引入了一种稳定的室温血型测定方法,使用工程红细胞. 这种基于纸张的新方法提供了快速,准确的ABO分组和抗体定位,这对于紧急情况下至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 输血医学 输血医学
- 临床诊断 临床诊断 临床诊断 临床诊断
背景情况:
- 目前的血液分型方法依赖于冷链依赖的试剂和手动解释,限制其在资源有限的环境中使用.
- 现有的技术涉及耗时的连续稀释和血结的主观视觉评估.
- 需要稳定,快速,客观的血液分类和抗体定位方法.
研究的目的:
- 开发一种独立于冷链的临床检测方法,用于精确的血液分类和抗体定位.
- 为了克服使用生物工程红细胞的传统方法的局限性.
- 在各种临床环境中实现快速可靠的输血决策.
主要方法:
- 开发了工程化的红血细胞膜,这些红血细胞膜缺乏血红蛋白,并保留了ABO抗原.
- 这些工程细胞被光纳米颗粒标记,并为室温稳定性而冷解.
- 一个基于纸张的设备通过光在大约8分钟内捕获和量化了血液凝结.
主要成果:
- 该试验表明,在室温下至少有2年的稳定性.
- 临床试验显示,与凝微柱测定相比,ABO类型的一致性为100%.
- 定量抗体标位与凝微柱测定有很强的一致性 (巧合率为80.75%, κ=0.8623).
结论:
- 一种新的,冷链独立的,基于纸张的测定方法可以同时进行ABO分组和定量抗体定位.
- 该试验提供临床级性能,支持可扩展的制造和标准化.
- 这项技术在紧急情况下和资源有限的情况下对输血决策具有重大潜力.
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