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一个AuPt纳米酶辅助的CRISPR/Cas12a系统用于视觉核酸检测病原体

Chenfei Zhao¹, Hangyu Guo¹, Jianai Chen¹

¹School of Life Sciences, Jilin University, Changchun, 130012, Jilin, China.

Analytical and bioanalytical chemistry

|February 5, 2026

概括

一种新的纳米粒子酶辅助CRISPR检测 (NACD) 试验提供了简单,灵敏和视觉检测土豆早期病由Alternaria solani引起. 这种现场诊断工具增强了土豆行业的疾病管理.

科学领域:

农业科学农业科学
生物技术是生物技术.
分子诊断学分子诊断

背景情况:

由于Alternaria solani引起的土豆早期腐烂,对土豆生产构成重大威胁.
目前的A. solani检测方法往往缺乏简单性和准确性.
需要快速的现场诊断工具,以有效管理疾病.

研究的目的:

开发一种新的,简单的,灵敏的,可视检测系统来检测Alternaria solani.
将CRISPR/Cas12a技术与纳米酶集成,以提高检测能力.
创建一个适合土豆行业的现场诊断工具.

主要方法:

开发一种金 (AuPt) 纳米酶辅助CRISPR/Cas12a系统,称为纳米粒子酶辅助CRISPR检测 (NACD) 试验.
利用CRISPR/Cas12a的精确目标识别和AuPt纳米酶的类似酶的活性.
整合了基于纸张的过器读数以进行裸眼视觉检测.

主要成果:

通过NACD测试,A. solani的敏感检测得到了实现,目标dDNA的限值为100副本/μL,基因组DNA的限值为10−3 ng/μL.
通过AuPt纳米酶介导的明显颜色变化获得视觉结果.
该试验显示高特异性,没有与其他常见的土豆病原体交叉反应.

结论:

NACD测定提供了一种简单,敏感和可视的方法来检测Alternaria solani.
它的现场适用性,由于基于过纸的读数,使其非常适合在土豆行业的实际疾病管理.
这种工具可以显著提高管理马早期病的效率.

关键词:

另一个是Alternaria solani.这就是CRISPR/Cas12a.纳米酶纳米酶是一种纳米酶.基于纸质的生物传感器.视觉化的可视化

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