骨髓介质干细胞改善间歇性多发性硬化症:在cuprizone诱导的小鼠中,定量小脑显微镜和功能分析
Maedeh Hashemi1, Iraj Ragerdi Kashani1, Parichehr Pasbakhsh1
1Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
概括
鼻内骨髓介质干细胞 (BMSC) 治疗改善了多发性硬化症 (MS) 的小鼠模型中的运动功能和保存的大脑结构. 间歇性暴露于cuprizone增强了BMSC神经保护和再生的有效性.
科学领域:
- 神经科学是一个神经科学.
- 再生医学是一种再生医学.
- 干细胞疗法是一种干细胞疗法.
背景情况:
- 多发性硬化症 (MS) 的特征是组织学变化和随后的行为缺陷.
- 骨髓介质干细胞 (BMSCs) 显示出对MS治疗的前景.
- 这项研究研究了间歇性铜诱导 (INT-CPZ) MS模型中的鼻内BMSC输送.
研究的目的:
- 评估BMSCs在改善小脑结构和运动功能的有效性,在MS的INT-CPZ小鼠模型.
- 为了比较BMSCs在连续和间歇性cuprizone暴露模型中的影响.
主要方法:
- 小鼠被分为五组:对照组,CPZ组,CPZ+BMSC组,INT-CPZ组和INT-CPZ+BMSC组.
- 在第4周和第10周,BMSCs被注射入鼻.
- 用梁行走,杆子和梯子阶段测试来评估运动性能;小脑结构被立体分析.
主要成果:
- BMSC治疗显著改善了运动协调和平衡,INT-CPZ+BMSC组表现出更好的表现.
- 立体分析显示,在BMSC治疗组中,皮质和白质的体积损失减少,Purkinje细胞数量增加.
- INT-CPZ+BMSC组表现出最显著的结构保存和功能恢复.
结论:
- 鼻内BMSC疗法在暴露于cuprizone的小脑结构和运动功能的小脑维护方面是有效的.
- 间歇性库普里松暴露会增强BMSC的疗效,这表明神经再生和神经保护机制得到了增强.
- 这项研究强调了BMSC鼻内输送作为MS的治疗策略的潜力.
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