相关实验视频

Updated: Feb 7, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

IFNγ通过STAT1异型特异性表观遗传和转录机制来塑造巨细胞的炎症反应

Mojoyinola Joanna Akagha¹, Grigorios Georgolopoulos², David Martin¹

¹Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, 1210, Austria.

|February 5, 2026

概括

在干扰素- (IFNγ) 调节后,STAT1异型差异调节巨细胞对病原体的反应. 由STAT1微调炎症和抗病毒免疫的异形特异性表观遗传修饰.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

干扰素- (IFNγ) 激活了巨细胞来防御病原体.
IFNγ还通过诸如染色体重塑等机制对巨细胞进行重新编程,以便在未来与它们相遇.
在这些IFNγ介导的过程中,STAT1及其异型是至关重要的.

研究的目的:

研究STAT1异型在IFNγ诱导的表观遗传调节和巨细胞调节中的独特作用.
了解STAT1异型如何影响巨细胞对病原体相关分子的转录反应.

主要方法:

综合表观基因组 (H3K27ac,H3K4me1的ChIP-seq) 和转录基因组 (RNA-seq) 分析.
利用基因改造小鼠的原发性巨细胞.
研究了STAT1异构体对基因组修饰和基因表达的特异性影响.

主要成果:

STAT1异型差异调节巨细胞对脂聚糖 (LPS) 后IFNγ调节的反应.
在调节区域中,STAT1异型表现出明显的能力,能够调解IFNγ诱导的H3K27乙化变化.
缺少C端交换激活域 (TAD) 的STAT1β异型不能调解IFNγ对LPS诱导的转录的抑制作用,但保留了协作活性.
依赖STAT1 C终端TAD的IFNγ减弱抗病毒基因诱导,抑制LPS诱导的负反循环,放大炎症反应.

结论:

在IFNγ驱动的巨细胞表观遗传调节中,STAT1异型具有不同的作用.
这些异形特异性功能对于塑造先天免疫力和控制炎症至关重要.
这些发现为管理巨细胞对病原体反应的分子机制提供了新的见解.

关键词:

H3K27ac 在线阅读 H3K4me1 的意思是什么?干扰素- γ 是一种干扰素.脂聚糖化物 (LPS) 是一种巨细胞的调节条件在RNA-seqqq.

更多相关视频

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

相关概念视频

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...

Inflammatory Response I: Vascular and Cellular

Inflammatory Response I: Vascular and Cellular

The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...

Eukaryotic Transcription Activators

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...