铁单原子纳米酶的pH可切换多酶活性使双级联催化成为强大的双模式生物传感
Huifang Zhang1,2, Yuhang Zhang2, Xiang Xu2
1College of Chemistry and Chemical Engineering, Key Laboratory of Jiangxi Province for Environment and Energy Catalysis, Nanchang University, Nanchang 330031, China.
Analytical chemistry
|February 6, 2026
概括
这项研究引入了可切换pH的单原子纳米酶,用于先进的生物传感. 这些纳米酶提供双级联催化,提高了农药检测的灵敏度和准确性.
科学领域:
- 生物模拟化学 生物模拟化学
- 纳米材料科学 科学 纳米材料科学
- 分析化学 分析化学
背景情况:
- 具有多个类似酶的活动的纳米酶为生物感知提供了协同效益.
- 控制纳米酶功能而不需要交叉反应,用于多模式传感是一个重大挑战.
研究的目的:
- 开发一个可切换pH的单原子纳米酶平台,用于先进的生物传感.
- 为了精确地操纵双模式传感应用的多酶活动.
主要方法:
- 细胞染色体c (Cyt c) 模板式热解产生具有Fe-N5部分的Fe单原子纳米酶 (FeSAN).
- 研究了依赖pH的氧化酶,过氧化酶,超氧化物转化酶和类似催化酶的活动.
- 使用了具有反向信号校正的色度和电化学发光检测系统.
主要成果:
- FeSAN表现出可切换pH的双级联催化活性,使得依赖酸的氧化和依赖的抗氧化.
- 该平台在酸性介质中实现了放大色度反应,并在性介质中抑制了电化学发光.
- 对有机杀虫剂检测的灵敏度增加了10倍,准确度提高了.
结论:
- 通过使用可切换pH的单原子纳米酶建立了先进生物感知的新型范式.
- 成功构建了一个双级联催化平台,用于双模式传感.
- 突出了精确操纵纳米酶功能的潜力,以应对复杂的分析挑战.
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