用Saccharomyces cerevisiae进行多维工程,以有效地生产网膜醇
Minxia Song1,2, Jiaheng Liu1,2, Xianhao Xu1,2
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Biotechnology journal
|February 6, 2026
概括
研究人员对酵母进行了改造,以产生高度的视网醇 (维生素A). 通过优化Saccharomyces cerevisiae中的关键酶和代谢途径,实现了创纪录的7.19g/L的网醇产量,提高了其工业应用的潜力.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 乙醇 (维生素A) 对视力,免疫力和皮肤健康至关重要.
- 麦芽菌 (Saccharomyces cerevisiae) 拥有用于网醇前体合成的美瓦酸途径.
- 代谢流失平衡限制了酵母酵母中的视网醇生产效率.
研究的目的:
- 为了增强Saccharomyces cerevisiae中的视网生物合成.
- 为了克服在醇生产中的代谢瓶和流量转移.
- 为了实现对潜在的工业规模扩大,实现高产量的网醇生产.
主要方法:
- 优化了关键酶的基因拷贝数量:格拉尼尔格拉尼尔二酸盐合成酶 (CrtE) 和β-胡卜素15,15'-二氧化酶 (Blh).
- 通过促进物替代 (PSPI1) 和分解,调节的烯合成酶 (ERG9) 表达.
- 实施了辅助性标记基因补充,以进一步提高产量.
主要成果:
- 优化CrtE和Blh基因拷贝数增加了72.0%的视网醇标位.
- 多维操纵ERG9表达和分离,提升位至1.41g/L.
- 在5L生物反应器中,最终的视网醇标位达到7.19g/L,这是S. cerevisiae的记录.
结论:
- 开发了一种有效的工程策略,用于在S. cerevisiae中生产高产量的视网醇.
- 已证明成功地将代谢流向重定向到视网醇合成.
- 为后续的工艺开发和扩大网醇生产奠定了基础.
相关概念视频
Production Efficiency
18.4K
Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
18.4K
What is Genetic Engineering?
80.3K
Overview
80.3K
Heat Engines
3.7K
A heat engine is a device used to extract heat from a source and then convert it into mechanical work used for various applications. For example, a steam engine on an old-style train can produce the work needed for driving the train.
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...
3.7K
Trophic Efficiency
25.2K
Trophic level transfer efficiency (TLTE) is a measure of the total energy transfer from one trophic level to the next. Due to extensive energy loss as metabolic heat, an average of only 10% of the original energy obtained is passed on to the next level. This pattern of energy loss severely limits the possible number of trophic levels in a food chain.
25.2K
Scalar Product (Dot Product)
27.7K
The scalar multiplication of two vectors is known as the scalar or dot product. As the name indicates, the scalar product of two vectors results in a number, that is, a scalar quantity. Scalar products are used to define work and energy relations. For example, the work that a force (a vector) performs on an object while causing its displacement (a vector) is defined as a scalar product of the force vector with the displacement vector.
The scalar product of two vectors is obtained by multiplying...
The scalar product of two vectors is obtained by multiplying...
27.7K
Vector Product (Cross Product)
28.1K
Vector multiplication of two vectors yields a vector product, with the magnitude equal to the product of the individual vectors multiplied by the sine of the angle between both the vectors and the direction perpendicular to both the individual vectors. As there are always two directions perpendicular to a given plane, one on each side, the direction of the vector product is governed by the right-hand thumb rule.
Consider the cross product of two vectors. Imagine rotating the first vector about...
Consider the cross product of two vectors. Imagine rotating the first vector about...
28.1K


