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硫甲醇的内部分子标记参考标准,用于通过喷射电气离子化轨道轨道质谱仪进行碎片特异同位素分析

Aoife Canavan¹, Christopher Dirr¹, Martin Elsner¹

¹TUM School of Natural Sciences, Department of Chemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Lichtenbergstraße 4, 85748 Garching, Germany.

Journal of the American Society for Mass Spectrometry

|February 6, 2026

概括

使用质谱学进行了硫甲醇 (SMX) 的稳定同位素分析. 这种方法量化了碎片特定的同位素特征,有助于对这种常见抗生素的环境命运研究.

科学领域:

环境化学环境化学
分析化学分析化学
同位素地球化学同位素地球化学

背景情况:

药品,包括诸如硫甲醇 (SMX) 等抗生素,在水生环境中普遍存在.
了解这些污染物的环境命运和降解途径至关重要.
稳定同位素分析为污染源和转化过程提供了洞察力.

研究的目的:

开发一种特定化合物的方法来分析SMX中的碳和稳定同位素.
为了使碎片特定的同位素分析 (Δδ13C和Δδ15N) 使用电喷射电离 (ESI) 轨道轨道质谱 (MS).
建立用于校准分析方法的参考标准.

主要方法:

综合SMX参考标准与特定的13C和15N标签.
准备含有标记和自然丰富的SMX的混合标准.
使用元素分析同位素比重质谱法 (EA-IRMS) 确定散装同位素值.
对ESI-Orbitrap-MS进行SMX碎片 (F92和F99) 的碎片特定同位素分析的校准和应用.

主要成果:

已建立的方法实现了高精度的碎片特定同位素分析:95%CI为0.7‰和3.4‰的δδ13C和δδ15N在F92,和1.3‰和2.9‰的δδ13C和δδ15N在F99.
证明了在SMX降解过程中量化同位素特征变化的原理证明.
验证了用于精确ESI-Orbitrap-MS校准的合成标准的使用.

结论:

开发的碎片特定同位素分析方法对于研究SMX环境命运是有效的.
这种技术为跟踪水生系统中抗生素降解和转化提供了强大的工具.
化合物特异性同位素分析有助于我们更好地了解制药污染及其对环境的影响.

关键词:

轨道飞行器-IRMS 在轨飞行器抗生素抗生素是一种抗生素.碰撞导致的碎片化.在分子内同位素的比率.同位素分离的分化方法同位素标准是同位素的标准.动态同位素效应的动力效应.参考材料是参考材料的参考材料.硫胺酸硫胺的使用方法

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