性-pH转移的豆蛋白分离物用于喷雾干燥的益生菌封装:洞察分子细胞壁相互作用和氧化保护
Akkaratch Rodklongtan1, Patharapim Laurujisawat1, Thatchawan Dumrongchai1
1Technological Innovation of probiotics and plant extracts for functional food special research unit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Food chemistry: X
|February 6, 2026
概括
转移的豆蛋白分离物 (pHMBPI) 在喷雾干燥和消化过程中显著改善益生菌的存活率. 这种新型封装剂提供了增强的稳定性和抗氧化特性,以更好地传递Limosilactobacillus reuteri KUB-AC5.5.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 益生菌由于热量和氧化应激导致喷雾干燥后活力较低.
- 有效的封装对于保持益生菌功能至关重要.
研究的目的:
- 评估性-pH转移的豆蛋白分离物 (pHMBPI) 作为Limosilactobacillus reuteri KUB-AC5.5的封装剂.
- 为了比较pHMBPI与豆蛋白分离物 (MBPI) 和乳清蛋白分离物 (WPI).
主要方法:
- 使用pHMBPI,MBPI和WPI进行Limosilactobacillus reuteri KUB-AC5的喷雾干燥.
- 物理性质 (溶解性,疏水性) 和抗氧化活性 (ABTS•+清理) 的评估.
- 在模拟消化过程中使用NMR和分子动力学模拟来评估细胞活力,膜完整性和稳定性.
主要成果:
- pHMBPI显示出卓越的溶解性,表面疏水性和抗氧化活性.
- pHMBPI封装导致细胞壁和膜损伤显著降低,可活细胞数量增加,生存率提高.
- 封装的益生菌通过模拟的胃和肠道消化保持了高活力,NMR证实减少了脂质过氧化.
结论:
- pHMBPI是益生菌的高效封装剂,增强热稳定性和抗氧化剂防御.
- 在pHMBPI组件和细菌细胞壁目标之间强大的静电相互作用有助于改善封装.
- pHMBPI为提供具有增强功能性质的可行的益生菌提供了一个有前途的战略.
关键词:
抗氧化剂蛋白质矩阵是一种抗氧化剂蛋白质矩阵.细胞壁结合蛋白质 细胞壁结合蛋白质利莫西拉ctobacillus reuteri KUB-AC5 的使用情况.膜脂质过氧化过氧化分子动态模拟分子动态模拟植物性封装剂是植物性封装剂热应力保护 热应力保护更多相关视频
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