增强DNA功能化碳纳米管的光学性能和稳定性,使用冷保护剂介导的冷化
Aceer Nadeem1,2, Aidan Kindopp1, Ella Junge1
1Department of Chemical Engineering, University of Rhode Island, Kingston, RI, 02881, United States.
概括
用葡萄糖和聚乙烯糖醇 (PEG) 结晶保护剂的冷化稳定了用于近红外光成像的单壁碳纳米管 (SWCNTs). 这种方法可以防止聚合,确保生物医学应用的可靠性能.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
背景情况:
- 与DNA功能化的单壁碳纳米管 (SWCNTs) 对于近红外 (NIR) 光生物传感至关重要.
- 储存期间DNA-SWCNTs的聚合会损害它们的光学性能和稳定性.
- 长期稳定性对于生物传感和成像中的可靠应用至关重要.
研究的目的:
- 增强DNA-SWCNTs的长期稳定性和室温复制.
- 评估各种冷保护剂在冷化后保护SWCNT光学性能方面的有效性.
- 评估生物医学应用中优化冷凍化的潜力.
主要方法:
- 研究的冷化与五种冷保护剂相结合:葡萄糖,砂糖,曼尼托尔,聚乙烯糖醇 (PEG) 和聚乙烯醇 (PVA).
- 评估了NIR光的保存和化和复制后聚合的预防.
- 使用小鼠巨细胞进行了体外研究,以评估细胞内光学性能.
主要成果:
- 葡萄糖和PEG,特别是80:20的比例,在保持NIR光和防止聚合方面表现最好.
- 用葡萄糖-PEG保护剂的冷化SWCNT保持了稳定的细胞内光学性能.
- 优化的冷化协议显著改善了保质期和可重复性.
结论:
- 使用特定的冷保护剂组合 (葡萄糖-PEG) 的优化冷化增强了DNA-SWCNT的稳定性和复制性.
- 这种方法支持长期储存,易于运输和可靠地在生物医学和临床环境中使用.
- 改进的SWCNT传感器保质期和可重复性为更广泛的应用铺平了道路.
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