在波式生物反应器中模拟和验证氧气运输:优化的实验性质量转移方法和新的拉蒂斯-博尔兹曼CFD方法
S Piontek1, J Fitschen1, C Weiland2
1Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany.
Frontiers in bioengineering and biotechnology
|February 6, 2026
概括
波式生物反应器的准确表征对于生物制药上游过程至关重要. 这项研究使用实验和CFD确定了体积质量转移系数 (kLa),优化了改善细胞培养性能的参数.
科学领域:
- 生物技术是生物技术.
- 生物工艺工程 生物工艺工程
- 细胞培养技术 细胞培养技术
背景情况:
- 波式生物反应器对于生物制药上游过程至关重要,弥合了摇瓶和箱之间的差距.
- 优化细胞培养性能需要准确地描述波式生物反应器,特别是对于需要更高质量转移的先进细胞系.
- 切割应力最小化和受控环境是波动生物反应堆的关键优势.
研究的目的:
- 通过实验测量和计算流体动力学 (CFD) 模拟来确定波浪生物反应器中的体积质量转移系数 (kLa).
- 为了将kLa与关键过程参数 (如摇角度,摇速率和填充体积) 相对应.
- 识别和验证优化过程参数,以提高细胞培养.
主要方法:
- 为了测量体积质量转移系数,开发了一种改进的实验方法.
- 使用M-Star CFD与拉蒂斯-博尔茨曼方法解决器进行了CFD模拟.
- 质量转移计算整合了希比的透理论,科尔摩戈罗夫尺度,弗里德尔的概念和表面更新模型,包括表面正常速度.
主要成果:
- 在10L和50L波式生物反应器上进行了实验和CFD分析.
- 确定了优化的工艺参数,导致生物栽培期间介质中溶解氧水平显著增加.
- 该研究表明,波浪生物反应器的特性和控制能力得到了改进.
结论:
- 这些发现可以更准确地预测工艺参数对波浪生物反应器性能的影响.
- 优化的波式生物反应器操作可以提高生物制药制造中的细胞培养效率和产品产量.
- 这项研究为生物工艺开发中的先进控制策略提供了基础.
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