急性木烟暴露与加速卵巢衰竭小鼠模型中的细胞特异性海马体转录基因反应有关
bioRxiv : the preprint server for biology
|February 6, 2026
概括
卵巢激素的下降可能会增加老年女性大脑对野火烟雾的脆弱性. 这项研究揭示了在加速卵巢衰竭的小鼠模型中,在木烟暴露后,海马体中细胞特异性基因表达变化.
科学领域:
- 环境健康 环境健康
- 神经科学是一个神经科学.
- 内分泌学 在内分泌学.
背景情况:
- 野火事件正在增加,对易受空气污染影响的老龄化人口构成风险.
- 更年期期间卵巢激素下降对大脑对空气污染的脆弱性的影响尚不清楚.
- 更年期涉及影响心血管,神经和免疫健康的生理变化.
研究的目的:
- 为了研究一个类似于经更年期的荷尔蒙状态是否会放大海马对急性木烟 (WS) 暴露的反应.
- 在雌性小鼠中模拟中度加速卵巢衰竭 (AOF),以研究激素对神经炎症的影响.
- 为了确定WS暴露后海马体中细胞类型特定的转录性改变.
主要方法:
- 利用一个卵巢完整的小鼠模型与加速卵巢衰竭 (AOF) 诱导的4-乙烯基环二氧化物 (VCD).
- 在连续两天内,每天暴露在过空气 (FA) 或木烟 (WS) 中的小鼠每天4小时.
- 采用空间转录学 (10x Visium) 和自动化的细胞类型注释来分析海马组织.
主要成果:
- 木烟暴露在各种海马细胞群体中诱导了明显的转录模式,包括星球细胞,神经元和寡细胞.
- 空间转录学确定了WS暴露后基因表达中的细胞类型特定变化.
- 该研究证实WS的组成包括微量金属和气体.
结论:
- 建立了一个细胞类型解决的转录框架,将木烟暴露与卵巢激素下降联系起来.
- 确定了潜在的细胞通路,有助于老年女性海马的脆弱性.
- 研究结果强调了环境污染物在荷尔蒙变化的背景下对神经生物学影响.
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