通过自适应性斜平面显微镜自适应性斜平面显微镜从微观到宏观的多功能高速体积成像
bioRxiv : the preprint server for biology
|February 6, 2026
概括
一个新的自适应斜平面显微镜 (OPM) 系统可以在大型生物样本中提供灵活,高速的4D成像. 这种先进的显微镜技术能够详细可视化复杂的多细胞系统中的动态过程.
科学领域:
- 先进的显微镜技术技术的显微镜技术.
- 生物医学成像学 生物医学成像学
- 神经科学研究研究的神经科学研究.
背景情况:
- 在复杂的多细胞系统中,对高速,大规模体积计记录的需求日益增加.
- 斜平面显微镜 (OPM) 提供实时4D显微镜,但在缩放超过1mm时面临限制.
- 目前的OPM方法与像活着的老鼠大脑这样的大型标本作斗争.
研究的目的:
- 开发一个自我适应的OPM系统,克服视野和分辨率的限制.
- 为了实现灵活,高速的体积成像在各种生物尺度.
- 在大型多细胞模型中促进动态过程的先进成像.
主要方法:
- 利用Abbe的正弦条件来实现自我适应的OPM.
- 实现可切换的成像分辨率,音量和速度.
- 实现快速光学扫描和远程聚焦,实现实时4D数据采集.
主要成果:
- 在一系列视野 (高达8毫米2) 和分辨率 (低至2.2微米3) 中表现出灵活性.
- 在斑马鱼 (1×0.4 mm2 FOV 5 Hz) 中启用全脑单个神经元体积成像.
- 在100 Hz的小鼠大脑 (>3×3 mm2 FOV) 中实现了毛细血管血细胞跟踪.
- 在被清除的小鼠大脑中展示了用于细胞下结构可视化的屏幕和变焦能力.
结论:
- 自适应的OPM提供了一个多功能平台,用于研究多细胞系统中的动态过程.
- 这项技术支持体内和固定,光学清除的样品.
- 为生物发现提供前所未有的大规模,高速体积成像.
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