选分子识别基于元素的SWCNT光学传感器用于炎症性细胞因子TNF-α
bioRxiv : the preprint server for biology
|February 6, 2026
概括
研究人员开发了使用单壁碳纳米管的新型体和抗体传感器,以检测血清中的瘤坏死因子-α (TNF-α). 一个经过修改的体传感器显示有选择性的TNF-α检测,这对于诊断炎症性疾病至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 免疫学 免疫学 免疫学
背景情况:
- 瘤坏死因子-α (TNF-α) 是一种关键的促炎细胞因子,与许多疾病有关.
- 精确量化TNF-α对于疾病诊断和监测治疗反应至关重要.
- 单壁碳纳米管 (SWCNT) 为生物传感应用提供独特的光发光特性.
研究的目的:
- 通过SWCNT信号转导来评估基于aptamer和抗体的TNF-α检测传感器.
- 为了优化像血清这样复杂的生物矩阵中的传感器性能.
- 确定潜在的诊断和研究应用的领先传感器结构.
主要方法:
- 开发和测试针对TNF-α的多个阿巴和抗体传感器结构.
- 利用SWCNT近红外光发光来进行信号传导.
- 在血清中评估传感器灵敏度和选择性,有或没有被动化和火器修改.
主要成果:
- 几种阿普坦和抗体传感器在生理范围内的血清中显示出敏感和选择性的TNF-α检测.
- 通过被动化和加入外源火器来提高传感器的性能.
- 一个用黑洞灭器修改的阿普坦酶序列,结合聚-L-lysine被动化,实现了选择性血清检测.
结论:
- 将分子识别元素翻译为新平台 (SWCNT) 和条件 (血清) 提出了挑战,但是可行的.
- 一个领先的传感器构造得到了验证,在血清中改进了以前失败的设计.
- 开发的传感器和评估框架对诊断和研究由炎症驱动的慢性疾病充满希望.
相关概念视频
Classification of Elements and Compounds
73.2K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond. Elements are classified as atomic or molecular based on the nature of their basic units.
Compounds are pure substances composed of two or more elements in fixed, definite proportions. Compounds are classified as ionic or molecular (covalent) based on the bonds...
Compounds are pure substances composed of two or more elements in fixed, definite proportions. Compounds are classified as ionic or molecular (covalent) based on the bonds...
73.2K
Elements and Compounds
105.0K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
105.0K
Factors Affecting α-Alkylation of Ketones: Choice of Base
3.6K
α-Alkylation of ketones is achieved in the presence of alkyl halides and a base. The reaction proceeds via the formation of an enolate ion followed by nucleophilic substitution. The choice of base employed is essential as it is the key factor in determining the reaction outcome.
The reaction involving bases like EtO− whose conjugate acid EtOH (pKa = 15.9) is stronger than the ketone (pKa = 19.2) results in an equilibrium mixture with higher ketone concentration. As a consequence,...
The reaction involving bases like EtO− whose conjugate acid EtOH (pKa = 15.9) is stronger than the ketone (pKa = 19.2) results in an equilibrium mixture with higher ketone concentration. As a consequence,...
3.6K
Periodic Classification of the Elements
59.2K
The periodic table arranges atoms based on increasing atomic number so that elements with the same chemical properties recur periodically. When their electron configurations are added to the table, a periodic recurrence of similar electron configurations in the outer shells of these elements is observed. Because they are in the outer shells of an atom, valence electrons play the most important role in chemical reactions. The outer electrons have the highest energy of the electrons in an atom...
59.2K
Molecular Compounds: Formulas and Nomenclature
55.9K
Molecular compounds or covalent compounds result when atoms share electrons to form covalent bonds. Since there is no electron transfer, molecular compounds do not contain ions; instead, they consist of discrete, neutral molecules.
55.9K
Key Elements for Plant Nutrition
24.3K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.3K


