终端分化流感特异效应记忆B细胞在活体减弱流感疫苗接种后循环
bioRxiv : the preprint server for biology
|February 6, 2026
概括
活体减弱型流感疫苗 (LAIV) 引起特定的B细胞,但与非活化疫苗不同,这些细胞不能预测抗体反应. 拉伊夫诱导终端分化的B细胞,不能被召回.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 细胞免疫学 细胞免疫学
背景情况:
- 活体减弱型流感疫苗 (LAIV) 是FDA批准的唯一一种粘膜流感疫苗.
- 目前缺乏针对LAIV的循环保护相关物质.
- 之前的研究发现了HA特异性IgD阴性B型记忆细胞,预测了无活化流感疫苗 (IIV) 后的反应.
研究的目的:
- 在LAIV之后,对循环的HA特异性IgD阴性记忆B细胞进行分析.
- 为了确定循环B细胞子集,这些子集可以预测LAIV后的抗体反应.
- 为了比较由LAIV与IIV诱导的B细胞反应.
主要方法:
- 在LAIV后的患者中分析循环HA特异性IgD阴性记忆B细胞.
- 使用色标记的血凝素 (HA) 抗原进行表型和转录分析.
- 监督和无监督分析以确定细胞子集并与抗体反应相关联.
主要成果:
- LAIV诱导循环的T-bet-阳性HA特异性IgD阴性B细胞,其表型与IIV之后的细胞相似.
- 这些T-贝特阳性细胞的数量与LAIV后的系统HA-IgG反应没有相关性.
- LAIV优先诱导HA特异性IgD阴性B细胞共同表达TBX21和Zeb2,表明终端分化.
- 在再次挑战时,LAIV诱导的T-贝特阳性B细胞无法被召回为分泌抗体的细胞.
结论:
- 在LAIV和IIV平台之间,循环HA特定的IgD阴性B细胞反应不同.
- LAIV诱导终端分化的B细胞,这些B细胞不能预测抗体水平或召回反应.
- 与LAIV疗效相关的新型循环B细胞标志物尚未确定.
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