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相关概念视频

Ribosomes01:27

Ribosomes

76.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
76.2K
Ribosomes01:27

Ribosomes

11.0K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
11.0K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.9K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.9K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

4.4K
4.4K
Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
Translation01:31

Translation

156.8K
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
156.8K

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相关实验视频

Updated: Feb 7, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

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核糖体蛋白bL27 保护转化核糖体从tmRNA-SmpBB中获得保护

Divyasorubini Seerpatham, George Wanes, Chathuri Pathirage

    bioRxiv : the preprint server for biology
    |February 6, 2026
    PubMed
    概括
    此摘要是机器生成的。

    细菌核糖体蛋白bL27意外地阻止了核糖体的救援机制,转译,阻止了蛋白质合成. 它的缺失严重影响细菌的生存能力,因为它破坏了这一至关重要的过程.

    更多相关视频

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    De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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    相关实验视频

    Last Updated: Feb 7, 2026

    Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
    11:19

    Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

    Published on: February 25, 2011

    20.3K
    Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
    06:58

    Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

    Published on: October 7, 2021

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    De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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    De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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    科学领域:

    • 分子生物学分子生物学
    • 生物化学 生物化学
    • 结构生物学 结构生物学

    背景情况:

    • 对于转化至关重要的细菌核糖体蛋白bL27的功能尚未完全理解.
    • bL27的氨基末端位于基转移中心附近,这表明它在核糖体功能中的作用.

    研究的目的:

    • 阐明细菌核糖体蛋白bL27在蛋白质合成中的作用.
    • 为了研究bL27与转译途径之间的相互作用.

    主要方法:

    • 用遗传学和生物化学来研究bL27的功能.
    • 用分子动力学模拟来分析ribosome上的bL27-tmRNA相互作用.

    主要成果:

    • 删除bL27基因导致细菌活力减少了1万倍.
    • 通过删除tmRNA基因,这是转译的关键组成部分,从而部分挽救了活力缺陷.
    • 分子动力学模拟表明bL27可以阻碍tmRNA在核糖体上的运动.

    结论:

    • 细菌核糖体蛋白bL27在防止转译干扰蛋白质合成方面发挥着至关重要的作用.
    • bL27的氨基末端作为一个守门员,抑制tmRNA在翻译过程中使tRNA结合受到硬质性阻碍.