Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

6.3K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
6.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
The Extracellular Matrix01:42

The Extracellular Matrix

89.2K
Overview
89.2K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

7.1K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
7.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Validation of the Standardized Outcomes in Nephrology - Life Participation (SONG-LP) Instrument in People Receiving Dialysis.

Kidney international reports·2026
Same author

A Humanized Knock-in Mouse Model of the PSEN1 V97L Mutation from a Major Chinese Alzheimer's Disease Pedigree Reveals Early Neuroinflammation.

Neuroscience bulletin·2026
Same author

Hyperactive BMP and Mechanosignaling Remodel Chromatin to Drive Aberrant Osteogenesis in FOP.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same author

O-SNAP uncovers nanoscale chromatin remodeling in dedifferentiation and stress responses.

Nature communications·2026
Same author

Reprogramming Dedifferentiation Regulatory Networks Preserves Human Chondrocyte Phenotypes.

bioRxiv : the preprint server for biology·2026
Same author

Engineering a Matrix-Preserving Vascular dECM Platform with Tunable Stiffness for <i>In Vitro</i> Vascular Remodeling.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Feb 7, 2026

Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
04:16

Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

Published on: August 5, 2021

2.7K

动态机械负载重编程阴囊细胞衍生的细胞外囊泡,以提高它们的再生能力.

Catherine Cheung, Zizhao Li, Sung-Han Jo

    bioRxiv : the preprint server for biology
    |February 6, 2026
    PubMed
    概括
    此摘要是机器生成的。

    生理力学机械负荷增加了阴茎纤维肌细胞衍生的细胞外囊泡 (MFC-EVs) 的产量和再生能力. 这些"机械启动"的EV被丰富了促进组织修复的蛋白质,为阴茎再生提供了一个有前途的治疗策略.

    更多相关视频

    Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
    06:28

    Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

    Published on: February 17, 2023

    1.6K
    Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs
    04:54

    Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

    Published on: May 3, 2024

    1.2K

    相关实验视频

    Last Updated: Feb 7, 2026

    Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
    04:16

    Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

    Published on: August 5, 2021

    2.7K
    Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
    06:28

    Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

    Published on: February 17, 2023

    1.6K
    Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs
    04:54

    Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

    Published on: May 3, 2024

    1.2K

    科学领域:

    • 生物材料科学 生物材料科学
    • 再生医学是一种再生医学.
    • 组织工程是组织工程.

    背景情况:

    • 半径对于膝关节功能至关重要,但其不良的愈合能力对受伤后的再生构成重大挑战.
    • 细胞外囊泡 (EVs) 显示出用于组织修复的治疗潜力,但在临床使用中产量和生物活性受到限制.
    • 了解如何增强EV生产和功能对于它们的治疗应用至关重要.

    研究的目的:

    • 研究动态机械负荷对阴茎纤维肌细胞衍生的EVs (MFC-EVs) 的产生,组成和功能的影响.
    • 确定机械刺激是否可以增强MFC-EVs的再生能力,以便在阴囊修复中潜在的治疗应用.

    主要方法:

    • 阴茎纤维粒细胞被培养并使用定制生物反应器进行生理相关的循环拉伸负荷.
    • 收集了电动汽车,并分析了它们的生产,大小,四氨酸表达和蛋白质货物.
    • 机械刺激的EVs对受体介质层细胞 (MSC) 的功能影响通过测量aggrecan表达来评估.

    主要成果:

    • 机械负荷通过ESCRT独立的途径显著增加了MFC-EVs的产生和分泌.
    • 机械启动的EV在MSC中增强了农草甘的表达,表明再生功能得到了改善.
    • 蛋白质组分析显示,装载的EV被丰富了细胞外基质,细胞骨蛋白和参与组织形态发生和软骨发育的途径.

    结论:

    • 生理学机械负荷可以提高MFC-EV的产量和再生能力.
    • 装载EV的增强生物活性归因于参与矩阵生产和组织发育的特定蛋白质的丰富.
    • 这项研究提供了一种可扩展的,生物启发的方法,用于设计强大的EV疗法,用于修复阴囊和更广泛的肌肉骨再生.