相关实验视频

Updated: Feb 7, 2026

High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

8.4K

在C.中模块基因标记. 伊莱根斯 (elegans) 是一个词

Adam Hefel, Kevin Kruse, Kaden Wall

    bioRxiv : the preprint server for biology
    |February 6, 2026
    PubMed
    概括

    我们介绍了PhIT,一种基于重组酶的方法,用于精确标记C. elegans中的蛋白质. 该系统克服了CRISPR的局限性,实现了模块化和无错误的基因标记,以改善蛋白质定位和表达研究.

    更多相关视频

    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit06:28

    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

    Published on: September 2, 2025

    830
    Basic Caenorhabditis elegans Methods: Synchronization and Observation11:34

    Basic Caenorhabditis elegans Methods: Synchronization and Observation

    Published on: June 10, 2012

    49.5K

    相关概念视频

    Proteins: From Genes to Degradation02:11

    Proteins: From Genes to Degradation

    Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
    Transcription is the synthesis of RNA...
    14.5K
    Tagging and Fusion Proteins01:24

    Tagging and Fusion Proteins

    Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
    8.5K
    Gene Flow02:39

    Gene Flow

    Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
    38.0K
    Gene Families01:57

    Gene Families

    Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
    Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
    10.0K
    Gene Therapy00:59

    Gene Therapy

    Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
    27.6K
    Gene Conversion02:08

    Gene Conversion

    Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
    10.7K