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相关概念视频

Reproductive Cloning01:27

Reproductive Cloning

32.8K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.8K
Transcription01:10

Transcription

156.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
156.8K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.0K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.0K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.8K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.8K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K

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相关实验视频

Updated: Feb 7, 2026

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
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Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

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从批量瘤测序中重建克隆解决的转录程序.

Jiaying Lai, Yi Yang, Kathleen Noller

    bioRxiv : the preprint server for biology
    |February 6, 2026
    PubMed
    概括
    此摘要是机器生成的。

    PICTographPlus通过整合DNA推断瘤进化来从大量RNA测序数据中重建克隆特异性基因表达. 这种方法将转录程序映射到特定的进化过渡,揭示了癌症克隆动态.

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    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
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    Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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    相关实验视频

    Last Updated: Feb 7, 2026

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    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
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    Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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    科学领域:

    • 基因组学就是基因组学.
    • 计算生物学 计算生物学
    • 癌症研究 癌症研究

    背景情况:

    • 瘤由具有模糊进化结构的相关克隆和在批量测序数据中的克隆特异性转录程序组成.
    • 从大量DNA测序推断出克隆架构是可能的,但从大量RNA-seq中恢复克隆解析的转录程序仍然具有挑战性.

    研究的目的:

    • 开发一个计算框架,PICTographPlus,通过将DNA推断的克隆基因基因与大量RNA-seq.集成来重建克隆解析的转录组.
    • 为了使克隆特异性基因表达的推断和特定进化过渡的途径收益和损失的局部化.

    主要方法:

    • 开发了PICTographPlus,这是一个概率框架,使用了基因结构规则化的混合模型.
    • 集成的DNA推断的克隆基因与大量的RNA-seq数据.
    • 使用单细胞衍生克隆结构和模拟的散装混合物在不同瘤纯度和采样密度上进行验证.

    主要成果:

    • 证明了克隆级基因组调节的强大恢复.
    • 应用于肺癌和胰腺癌队列,揭示了克隆受限的转录程序.
    • 确定了转录程序,瘤抑制剂损失和转移性进展之间的关联.

    结论:

    • PICTographPlus将批量测试转化为进化,克隆解析的转录地图.
    • 允许对瘤进化和转录程序进行回顾性和队列规模的分析,而无需专门的实验数据.
    • 为了解癌症异质性和进展提供了一个强大的工具.