在地幔细胞淋巴瘤中发现系统的融合转录,使用长读测序测序
bioRxiv : the preprint server for biology
|February 6, 2026
概括
研究人员使用Iso Sequencing在地幔细胞淋巴瘤中确定了新的融合转录,包括长非编码RNA融合. 这一发现突显了癌症转录组的复杂性,并有助于找到新的治疗点.
科学领域:
- 基因组学就是基因组学.
- 文字转录学 (Transcriptomics) 是一个学科.
- 癌症生物学 癌症生物学
背景情况:
- 来自不同基因的融合转录对于识别癌症生物标志物和治疗点至关重要.
- 在RNA水平上检测所有融合转录对于癌症研究至关重要.
研究的目的:
- 使用长读RNA测序,识别和描述地幔细胞淋巴瘤 (MCL) 中的融合转录.
- 研究新的融合转录,包括涉及长非编码RNA的转录.
主要方法:
- 在MCL细胞系上使用IsoSequencing (第三代长读RNA测序).
- 使用直角技术验证已识别的聚变转录.
- 研究了CPSF73/CPSF3抑制对特定聚变转录的作用.
主要成果:
- 揭示了广泛的转录多样性和MCL中的众多新的转录.
- 确定了几种新的融合转录,其中一些是MCL转录组中最长的.
- 已验证的已知 (CTBS::GNG5) 和新的融合转录 (RBM15::LAMTOR5:AS),包括一个涉及长非编码RNA的转录.
- 在接受CPSF73/CPSF3抑制剂JT-607治疗时观察到RBM15::LAMTOR5:AS的表达增加.
结论:
- 该研究确定了MCL中的新型融合转录 (RBM15::LAMTOR5:AS) 和许多其他融合转录.
- 这些发现强调了全面核聚变转录识别的公正方法的重要性.
- 发现的融合转录有助于理解癌症和正常生物学中的转录组复杂性.
相关概念视频
Transcription
156.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
156.8K
Nuclear Fusion
33.9K
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
33.9K
Master Transcription Regulators
7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Eukaryotic Transcription Inhibitors
11.0K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.0K
Uncertainty in Measurement: Reading Instruments
53.1K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
53.1K
Transcription Elongation Factors
14.0K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.0K


