早期接触PFAS会破坏斑马鱼胚胎的神经肌肉发育
bioRxiv : the preprint server for biology
|February 6, 2026
概括
暴露于和多醇基物质 (PFAS),特别是酸 (PFOA),通过改变基因表达和染色体组织来破坏斑马鱼的早期发育. 这导致幼虫的焦虑行为,将分子变化与生物体水平的结果联系起来.
科学领域:
- 发展生物学 发展生物学
- 环境毒理学环境毒理学
- 分子生物学分子生物学
背景情况:
- 开发对环境线索敏感,由于其持久性和生物积累,和多基物质 (PFAS) 引起了重大关注.
- 暴露于PFAS与发育问题有关,但潜在的细胞和分子机制仍然不清楚.
- 斑马鱼胚胎为研究环境污染物对早期发育的影响提供了一个有价值的模型.
研究的目的:
- 研究一种常见的PFAS,即 perfluorooctanoic acid (PFOA) 如何影响斑马鱼的早期胚胎发生.
- 探索PFOA在机体生成过程中的暴露引起的分子和细胞干扰.
- 评估PFOA暴露对后代发育的跨代影响.
主要方法:
- 斑马鱼胚胎暴露于低剂量和高剂量的PFOA.
- 分析是在受精后24小时 (hpf) 进行的,这是器官生成的关键阶段.
- 进行了单核RNA测序,以确定对PFOA的反应中的分子变化.
主要成果:
- 在分子层面上,PFOA暴露显著影响了神经元和肌肉组织的发育.
- 在暴露于PFOA的斑马鱼幼虫中观察到类似焦虑的行为表型.
- 暴露于PFOA改变了发育组织中的基因表达模式和染色质组织.
结论:
- PFAS,特别是PFOA,可以通过改变基因表达和染色体组织来破坏关键的发育过程.
- 由PFOA引起的早期分子干扰可以导致发育中的生物体的可观察到的行为缺陷.
- 这项研究提供了机制性的洞察力,了解像PFAS这样的环境污染物如何对发展产生负面影响并导致长期后果.
相关概念视频
01:18The Muscular System
7.2K
The muscular system is essential to the body's overall structure and function, playing a crucial role in movement, stability, and internal processes. It consists of three distinct types of muscle tissue: the skeletal, the smooth, and the cardiac muscles.
7.2K
01:15Development of the Sexual Organs in the Embryo and Fetus
3.8K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
3.8K
01:21Satellite Stem Cells and Muscular Dystrophy
2.4K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.4K
02:33Seed Structure and Early Development of the Sporophyte
31.2K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
31.2K
01:43Sustainable Development
15.2K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
15.2K
01:15Development of the Lymphatic System
2.1K
The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
2.1K