一个简化的CRISPRa架构与双极II/双极III促销器和优化的scRNA实现了强大的和可调节的基因激活
Dylan Gallo1,2, Martine Bes1,2, Thibault Mounier1,2
1CIRAD, UMR AGAP Institut, Montpellier, France.
The CRISPR journal
|February 6, 2026
概括
研究人员为植物开发了一个新的CRISPR激活 (CRISPRa) 平台,简化了基因上调. 这种精简的系统实现了强大的基因激活,为植物中的基因工程和合成生物学应用提供了灵活的工具.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因编辑技术 基因编辑技术
- 合成生物学 合成生物学
背景情况:
- 克里斯普尔激活 (CRISPRa) 是一种用于基因上调的强大工具.
- 现有的植物CRISPRa系统可能很复杂,很难与CRISPR干扰 (CRISPRi) 集成.
研究的目的:
- 开发一个精简和灵活的CRISPRa平台,用于植物中强大的基因激活.
- 为了对不同的引导RNA支架,效应蛋白和促进剂进行基准测试,以获得最佳的植物CRISPRa功能.
主要方法:
- 开发一种新型的单导向RNA (sgRNA) 架构,在四旋翼中使用MS2体,由复合Pol II/Pol III促进体驱动.
- 与gR2.0和SunTag等现有结构进行基准测试,使用双露西法酶记者测试.
- 在大米原生质中测试基因诱导的内源基因.
主要成果:
- 新型sgRNA支架表现出卓越的效率,达到最小的35S促销者的100倍激活.
- 在原始质细胞试验中观察到三种内源性大米基因的诱导率高达215倍.
- 具有3'体或过多拷贝数量的基架RNA被证明是无效的.
结论:
- 开发的模块化CRISPRa平台使米原生质中可调节的基因调控成为可能.
- 该系统为植物的高通量查和合成基因电路原型设计提供了实用的基础.
- 该平台的设计预计将在各种物种之间广泛可移植,用于基于CRISPR的转录模拟.
更多相关视频
06:47Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products
Published on: December 5, 2020
7.3K
08:31Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
Published on: March 29, 2019
30.7K
相关概念视频
The Eukaryotic Promoter Region
18.9K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.9K
LTR Retrotransposons
19.8K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
19.8K
Streamlines, Streaklines, and Pathlines
2.0K
A streamline represents the trajectory that is always tangent to the fluid's velocity vector at any given point. The velocity of a fluid particle is always directed along the streamline, ensuring the particle continuously follows the streamline's path. Streamlines are particularly useful for visualizing the overall direction of flow in a fluid system, and they provide an instantaneous representation of the flow's velocity field. In steady flow, where conditions do not change over...
2.0K
Polymer Classification: Architecture
3.9K
Polymers are classified as linear or branched on the basis of their chain architecture. The polymer chains in linear polymers have a long chain-like structure with minimal to no branching at all. Even if a polymer features large substituent groups on the monomer, which appear as branches to the skeleton, it is not considered a branched polymer. A branched polymer contains secondary polymer chains that arise from the main polymer chain. The branching occurs when the polymer growth shifts from...
3.9K
Organization of Genes
73.6K
Overview
73.6K
Gene Therapy
27.6K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.6K
