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BdAreA调节代谢,应激反应和Botryosphaeria dothidea中的毒性
Dewan Zhang1, Chunmei Han1, Na Liu1
1Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, Qingdao Engineering Research Center of Precise Control of Fruit and Vegetable Pests, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
概括
GATA转录因子BdAreA调节了Botryosphaeria dothidea中的代谢,生长和病原性. 这项研究揭示了它在控制压力反应中的作用,并将代谢与MAPK信号传递联系起来.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 植物病理学 植物病理学
背景情况:
- AreA是真菌中代谢的关键调节者.
- 在真菌物种中存在AreaA的功能分歧.
- 植物菌 (Botryosphaeria dothidea) 是导致果戒指腐烂的原因.
研究的目的:
- 描述B.dothidea.中的BdAreA的类功能.
- 研究BdAreA在代谢,生长,发育,致病性和应激反应中的作用.
- 探索代谢与MAPK信号传递之间的联系.
主要方法:
- 在B. dothidea.中针对BdAreA的向基因淘汰.
- 对ΔBdAreA突变体的表型分析.
- 实时定量PCR (RT-qPCR) 和酶活性测定.
- 对BdHog1表达和酸化的分析.
主要成果:
- ΔBdAreA突变显示出抑制的结合,降低的致病性,以及对氧化应激和植物素的敏感性.
- BdAreA积极调节缩酶 (BdNR) 和缩酶 (BdNIR) 的表达和活性.
- BdAreA负面调节BdHog1的表达和酸化,将代谢与MAPK信号连接起来.
结论:
- BdAreA是B. dothidea多个过程的主调节者,包括生长,发育,致病性,应激反应和代谢.
- 这项研究为代谢途径和MAPK信号级联之间的相互作用提供了新的见解.
- 了解BdAreA的功能对于管理B. dothidea感染至关重要.
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