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Ufd2p通过在半分裂过程中破坏Top2p的稳定性来促进高效的交叉形成

Taicong Tan¹, Yanan Zhao¹, Yinghong Chen^1,2

¹Institute of Reproductive Health and Perinatology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.

Proceedings of the National Academy of Sciences of the United States of America

|February 6, 2026

概括

E3 泛基因酶 Ufd2p 通过向 Topoisomerase II (Top2p) 进行降解来调节化过程中的交叉 (CO) 形成. 这样可以保持超级卷的稳态,确保精确的染色体分离和遗传多样性.

科学领域:

遗传学遗传学是一个
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

介质变化中的交叉 (CO) 形成对于精确的染色体分离和遗传多样性至关重要.
控制二氧化碳数量和分布的分子机制尚未完全理解.
无素-蛋白酶体系统与CO调节有关,但具体因素仍未定义.

研究的目的:

为了确定二氧化碳在变化过程中形成的新型调节剂.
阐明Ufd2p影响CO频率和分布的分子机制.
调查UBE4B在物种间CO形成中的保留作用.

主要方法:

在*Saccharomyces cerevisiae*中进行了聚焦基因查,以确定CO调节剂.
综合的多学分析,以确定Ufd2p的目标.
生物化学测定以评估无处不在和蛋白质体降解.
在酵母和哺乳动物细胞中进行功能测试,以确认保留的作用.

主要成果:

Ufd2p被确定为促进高效的二氧化碳形成的关键调节剂.
删除*UFD2*通过增强CO干扰来降低CO频率.
Ufd2p的目标是Topoisomerase II (Top2p),用于无处不在和降解.
在*UFD2*删除细胞中的Top2p积累导致了DNA负超线圈的过度分辨率和减少的CO数量.
哺乳动物UBE4B通过调节TOP2A依赖的超动力学来证明在CO形成中起着保留作用.
在*UFD2*删除酵母细胞中,UBE4B恢复了CO形成和介质性进展的表达.

结论:

Ufd2p 是一种介质交叉形成的新型调节剂.
Ufd2p-Top2p轴控制着DNA超级卷的稳态,影响CO的频率和分布.
这种机制在不同物种中得到保护,这突显了超级卷调节在半变异过程中的基本重要性.

关键词:

在Top2p上.Ufd2pp 在线播放交叉干扰是一种交叉干扰.介质性重组是介质性的重组.无处不在的合酶

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