基于液体染色学-双重质谱的尿路类固醇分析,应用于初级阿尔多斯特症诊断
Jian Zhong1, Xiaoli Ma1, Danchen Wang1
1Department of Laboratory Medicine; Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.
概括
一种新的液体染色学-并联质谱法 (LC-MS/MS) 方法能够快速,准确地进行尿路类固醇分析,用于诊断原发性阿尔多斯特主义 (PA). 这种高效的技术简化了样本准备,并提高了内分泌疾病的诊断能力.
科学领域:
- 内分泌学和代谢性疾病.
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 类固醇激素对于生理稳定和诊断诸如原发性阿尔多斯特隆症 (PA) 等疾病至关重要.
- 目前的尿路类固醇分析方法在分析剂覆盖率和样本准备效率方面存在局限性.
- 需要改进液体染色学-并联质谱法 (LC-MS/MS) 方法,以进行全面的类固醇分析.
研究的目的:
- 开发和验证一种新的LC-MS/MS方法,用于同时量化35种尿路类固醇激素.
- 建立一个高效的样品预处理协议,用于多类固醇分析.
- 评估用于诊断PA的开发方法的临床实用性.
主要方法:
- 建立了一个快速的LC-MS/MS方法,在25分钟的运行时间内量化尿液中的35种类固醇激素.
- 尿样在室温下经过高效的酶性水解,使用转基因β-glucuronidase.
- 使用沃特斯UPLC-TQ-SMS/MS系统系统地评估了线性,回收和精度的方法性能.
主要成果:
- 该方法表现出极好的线性,恢复率 (80.0120.6%) 和可接受的测试内和测试间精度 (CVs 1.8619.75%).
- 与非PA患者相比,患有PA的患者显示阿尔多和相关代谢物水平明显升高.
- 综合类固醇分析实现了PA的高诊断性能,AUC为0.914,灵敏度为0.87,特异性为0.88.
结论:
- 一种技术先进的LC-MS/MS方法与高效的酶性水解成功建立了精确的尿路类固醇量化.
- 这种方法可以进行多类固醇分析,以改善内分泌疾病的诊断.
- 开发的方法为临床诊断原发性阿尔多斯特主义提供了有价值的工具.
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