相关实验视频
Updated: Feb 8, 2026

10:20
Generation of a Human iPSC-Based Blood-Brain Barrier Chip
Published on: March 2, 2020
13.6K
通过新的中等尾酒LACID产生高质量的猪IPSC
Bingbo Shi1, Jiajun Li2, Xiaomin Wang3
1Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong, China.
Stem cell reports
|February 6, 2026
概括
研究人员开发了一种新方法,用于创建改善的猪诱导多能干细胞 (piPSCs),用于疾病建模和再生医学. 这些piPSC适合无料培养,可用于核转移以产生胚芽细胞.
科学领域:
- 干细胞生物学 干细胞生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 猪是疾病研究,异种移植和器官生成的关键模型.
- 产生具有理想属性的猪诱导多能干细胞 (piPSCs),如无料培养和发展潜力,一直具有挑战性.
研究的目的:
- 开发一种改进的策略,用于生成符合先进研究应用的关键标准的piPSC.
- 建立适合无料培养的piPSC,强大的发育潜力和核转移介导的胚胎细胞发育.
主要方法:
- 在猪重编程过程中利用化学定义的介质3来促进类似上皮质的殖民地形成.
- 在随后的重编程步骤中使用了新的媒介尾酒,LACID.
- 在无料条件下培养piPSC,并评估它们的多能性和发育能力.
主要成果:
- 成功生成了具有平面形态和无料培养能力的piPSC.
- 证明了强大的瘤和状体的形成,以及嵌合体细胞囊的产生.
- 通过生成的piPSCs展示了成功的CRISPR-Cas9编辑和核转移后的胚胎细胞发育.
结论:
- 新成立的LACID piPSC具有关键特征,使其成为再生医学应用的理想选择.
- 开发的方法在产生功能性piPSC方面取得了重大进展.
- 未来的改进可能会导致生成天真或全能猪干细胞.
相关概念视频
Quality Control
2.7K
Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
2.7K
Quality Assurance
2.4K
Quality assurance is the overarching term used to describe the activities employed to ensure the proper performance of a system. These activities can be classified into three categories: quality control, quality assessment, and internal corrective measures. Typically, these activities work cyclically: quality control is performed before and during the analysis, while quality assessment occurs during and after the investigation. Internal corrective measures are implemented based on the findings...
2.4K
Quality of Water
573
In concrete preparation, the quality of water is paramount as it affects the strength and durability of the concrete. Potable water is usually preferred; however, it must not have excessive sodium or potassium to prevent compromising the concrete's integrity. Water quality is typically evaluated based on impurities such as dissolved solids, chlorides, and sulfates, and its pH value is ideally between 6 and 8. Even slightly acidic natural water may be acceptable unless it contains harmful...
573
Pulse amplitude and quality
3.2K
Pulse amplitude is a crucial indicator of cardiac health because it provides valuable insights into the strength of left ventricular contractions and the overall uniformity of blood circulation within the vasculature. The strength of the pulse is directly related to the force with which the heart contracts and the volume of blood being pumped.
A weak or absent pulse may indicate reduced cardiac output or poor left ventricular contraction, which can be signs of cardiovascular dysfunction or...
A weak or absent pulse may indicate reduced cardiac output or poor left ventricular contraction, which can be signs of cardiovascular dysfunction or...
3.2K
Testing Water Quality
394
When the quality of water for concrete preparation is uncertain, its impact on the setting time of cement and compressive strength of mortar is assessed by comparison with de-ionized or distilled water benchmarks. American Society for Testing and Materials (ASTM) C1602 requires the setting times to be within 90 minutes of the control, British Standard (BS) 3146:1980 allows a 30-minute variance in the initial setting, while British Standards European Norm (BS EN) 1008 specifies initial setting...
394
Protein Folding Quality Check in the RER
5.3K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
5.3K

