来自Celastrus orbiculatus茎的生物活性化合物素通过CA2抑制抑制宫癌:桥梁基础研究和临床应用的翻译研究
Jue Chen1, MengKe Wu2, RongRong Zhang3
1Department of Oncology, Affiliated Hospital of Yangzhou University, Yangzhou 225001, China; Department of integrated Traditional Chinese and Western medicine, Medical College of Yangzhou University, Yangzhou 225001, China; The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the National Administration of Traditional Chinese Medicine, Yangzhou 225001, China; Jiangsu Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Geriatric Diseases, Yangzhou 225001, China; Taizhou Jiangyan Hospital of Traditional Chinese Medicine, Affiliated Hospital of Yangzhou University, Jiangyan District, Taizhou 225500, China.
含有黄素的 Celastrus orbiculatus 茎提取物通过向 CA2 蛋白来抑制子宫癌. 这种传统草药在改善无病生存和减少瘤生长方面表现有前途.
科学领域:
- 植物化学 植物化学
- 在瘤学瘤学.
- 生物信息学是一种生物信息学.
背景情况:
- 塞拉斯特 (Celastrus orbiculatus Thunb.) 已经成为一个常见的动物. 是一种传统的中国草药,具有已知的抗瘤特性.
- 它在治疗子宫癌的具体机制仍然没有被阐明.
- 这项研究研究了其在宫癌治疗中的潜力.
研究的目的:
- 阐明黄素的机制,这是Celastrus orbiculatus茎的关键组成部分,在抑制宫癌方面.
- 为了识别和验证黄素的抗癌作用中涉及的目标蛋白碳酸无水酶II (CA2).
- 以实验验证的回顾性临床发现为基础.
主要方法:
- 对Celastrus orbiculatus茎的回顾性临床分析与化学放射治疗相结合.
- 液体染色学-质谱学 (LC-MS) 用于活性成分的识别.
- 使用公共数据库 (TCGA) 的生物信息分析和细胞系中的实验验证以及小鼠异种移植模型.
主要成果:
- Celastrus orbiculatus茎与西斯普拉丁结合,改善了宫癌患者的无病生存率.
- 素被确定为主要活性成分,向CA2,该CA2在宫癌细胞中过度表达.
- 素对CA2的调节抑制了癌细胞的增殖,入侵,促进了细胞亡,并在体内抑制了瘤的生长.
结论:
- 丁醇是Celastrus orbiculatus茎中的主要生物活性化合物,负责宫癌抑制.
- CA2是宫癌中过度表达的关键标蛋白,促进了扩散和入侵.
- 向CA2用黄素提供了宫癌的潜在治疗策略.
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