来自三种药库中的多糖体的结构特征,生物活性和结构-活性关系 Polygonatum物种使用超声波辅助酶提取的酶提取方法
Wenjing Zhang1, Nuoyan Yang1, Shichao Ma1
1College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
International journal of biological macromolecules
|February 6, 2026
概括
在50分钟的超声波辅助酶化水解中,可以从三种多角动物中最佳地提取生物活性多糖,与95分钟相比,显示出优异的抗氧化,降糖,抗瘤和免疫调节作用. 这凸显了水解持续时间对黄多糖生物活性的重要性.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 生物化学 生物化学
背景情况:
- 黄金 (Polygonati Rhizoma),包括Polygonatum sibiricum,P. kingianum和P. cyrtonema,是一种传统的中国医学.
- 功能性多糖是黄金中的关键生物活性成分,具有潜在的健康益处.
- 优化提取方法对于最大限度地提高这些多糖的治疗潜力至关重要.
研究的目的:
- 为了比较从三种Polygonatum物种中提取的功能性多糖的生物活性,使用不同的超声波辅助酶解水解持续时间.
- 描述提取的多糖的主要结构,并将其与生物活性相关联.
- 确定最佳的水解持续时间,以最大限度地提高多糖生物活性.
主要方法:
- 使用超声波辅助的酶化水解来从Polygonatum物种中提取多糖.
- 六种同质的多糖类 (PSP50N,PSP95N,PKP50N,PKP95N,PCP50N,PCP95N) 进行了净化.
- 生物活性测定包括抗氧化剂,降糖,抗瘤和免疫调节评估.
- 结构特征包括分子量,果糖含量和链形状分析.
- 部分最小平方 (PLS) 和权TOPSIS被用于数据分析.
主要成果:
- 经过50分钟的水解提取的多糖体表现出明显高于95分钟提取的多糖体的生物活性.
- 这三个Polygonatum物种都表现出可比的疗效概况,但在效力上有所不同.
- PSP95N显示出强大的抗瘤活性,而PSP50N则表现出更强的免疫调节作用.
- 分子重量和果糖含量被确定为影响生物活性的关键结构因素,基于水解持续时间.
- 证实高生物活性的最佳提取持续时间为50分钟,生物活性排名:PSP50N > PCP50N > PKP50N > PCP95N > PSP95N > PKP95N.
结论:
- 超声波辅助酶性水解的持续时间显著影响黄多糖的生物活性.
- 50分钟是最大限度地提高这些三种Polygonatum物种中的多糖的综合生物活性的最佳水解持续时间.
- 了解结构-活性关系为选择Polygonatum物种和优化临床应用的提取提供了科学基础.
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