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在肺腺癌中测定c-Met免疫组合化学试验的评分可靠性

Yang Zhou¹, Junyi Pang², Yumeng Cai²

¹Department of Pathology, Peking Union Medical College, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, 100730, Beijing, China; Department of Pathology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, 102218, Beijing, China.

Laboratory investigation; a journal of technical methods and pathology

|February 6, 2026

概括

五个c-Met免疫组织化学 (IHC) 测定显示可靠的对应性检测c-Met过度表达在肺腺癌. 标准化评分,如临床评分2+/3+或H评分≥150,确保MET抑制剂治疗选择的诊断一致性.

科学领域:

在瘤学瘤学.
分子病理学分子病理学
生物标志物发现发现

背景情况:

c-Met过度表达是肺腺癌中MET抑制剂治疗的潜在生物标志物.
需要澄清c-Met免疫组织化学 (IHC) 测定和评分系统的可靠性和一致性.

研究的目的:

在肺腺癌样本中评估五种c-Met IHC测定和评分方法的一致性和可靠性.
为了比较不同c-Met IHC抗体和评分系统与参考标准的性能.

主要方法:

使用五种c-Met IHC试验 (SP44_R,SP44_Z,D1C2,LBP4-C-MET,811B7F4) 对150个肺腺癌样本进行了回顾性分析.
标本使用H分数,临床分数和2级分类来得分;MET变化通过107个样本的下一代测序来评估.
测试间的可靠性使用Intraclass关联系数进行评估,使用肯德尔的W和弗莱斯的 κ统计数据评估一致性.

主要成果:

在五个c-Met IHC试验中观察到H分数的优异的试验间可靠性 (ICC=0.953).
SP44_Z与SP44_R (ρ=0.851) 相关性最高,这是临床试验中常用的抗体.
两级H评分和临床评分 (κ范围:0.944-0.986) 几乎完全一致,而IHCH评分和RNA表达之间的相关性很弱 (ρ=0.159-0.349).

结论:

五个c-Met IHC试验在检测c-Met过度表达方面显示出中度至强度的一致性,SP44_R,SP44_Z,LBP4-C-MET和811B7F4的性能可靠.
临床评分 (2+/3+) 或H评分 (≥150) 提供了高的诊断一致性,支持使用多个验证的IHC测定用于肺腺癌中的c-Met评估.
D1C2对临床评分3+的一致性较小,这表明特定评分标准的潜在限制.

关键词:

临床评分临床评分在H分数中,得分为H.非小细胞肺癌是非小细胞肺癌.可靠性可靠性可靠性在c-Met Met中使用.

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