在裂变酵母中,TORC2协调MBF-依赖转录并抑制DNA复制应激期间的氧化应激反应
Adiel Cohen1, Ava Mouzon2, Uri Sprecher2
1Department of Natural Sciences, The Open University of Israel, University Road 1, 4353701, Ranana, Israel.
The Journal of biological chemistry
|February 6, 2026
概括
拉帕米辛复合体2 (TORC2) 的目标通过调节RNA聚合酶II.通过调节复制应激过程中确保了适当的基因激活. 此外,TORC2还可以防止Schizosaccharomyces pombe.中的不必要的氧化应激反应.
科学领域:
- 细胞生物学 细胞生物学
- 分子遗传学 分子遗传学
- 应激反应路径 应激反应路径
背景情况:
- 拉帕素 (TOR) 激酶的向对生长,新陈代谢和应激适应至关重要.
- 在Schizosaccharomyces pombe中,TOR复合体2 (TORC2) 对于在压力下生存至关重要,包括复制压力.
- MluI结合因子 (MBF) 复合体控制细胞周期进展和DNA复制应激反应.
研究的目的:
- 研究TORC2在复制压力期间调节基因转录中的作用.
- 阐明TORC2如何影响MBF依赖基因中的RNA聚合酶II动态.
- 为了确定TORC2是否影响其他应激反应途径,例如氧化应激.
主要方法:
- 在MBF促进体和编码区域对RNA聚合酶II (Pol II) 启动和延长形式的分析.
- 在复制应激下评估TORC2缺乏细胞中的基因表达变化.
- 在TORC2突变细胞中测量反应性氧物种 (ROS) 水平.
主要成果:
- 在复制应力期间,TORC2对于MBF促进体中启动Pol II的积累至关重要.
- TORC2缺乏导致MBF基因体中异常积累延长的Pol II,损害基因诱导.
- 在复制应激下,TORC2突变细胞显示氧化应激基因的不适当上调和ROS水平的增加.
结论:
- 在复制压力期间,TORC2对于准确调节MBF依赖基因转录至关重要.
- TORC2 作用于平衡 Pol II 启动和延长,确保适当的基因激活.
- TORC2在抑制氧化应激通路的激活中发挥作用,防止在复制应激过程中ROS积累.
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