聚乙烯纳米塑料的吸收由积卵细胞复合体损害了卵细胞和胚胎的发育
Jiayi Yang1, Kaylee Nieuwland2, Nikita L Korpel3
1Institute for Risk Assessment Sciences, Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, the Netherlands; Farm Animal Health, Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, the Netherlands.
Reproductive toxicology (Elmsford, N.Y.)
|February 6, 2026
概括
纳米塑料 (NP) 可以进入卵巢并影响卵细胞的发育. 这项研究发现50nm聚烯纳米塑料损害了牛卵细胞成熟和胚胎发育,但没有发生重大转录基因变化.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
背景情况:
- 纳米塑料 (NP),颗粒<1微米,对健康构成潜在风险.
- 动物研究表明NP可以穿越生物障碍,到达女性生殖道和卵巢.
- 卵巢NP的存在可能会影响生殖结果,包括卵细胞的发育.
研究的目的:
- 研究聚烯 (PS) 纳米塑料对牛卵细胞和胚胎发育的吸收和影响.
- 使用牛体外受精 (IVF) 模型来评估NP的影响.
- 使用RNA测序来探索潜在的分子机制.
主要方法:
- 在体外成熟过程中,牛累积卵细胞复合体 (COC) 暴露于50nm或200nm的PS-NP.
- 暴露后,COCs接受了试管婴儿和胚胎培养.
- 使用共聚焦显微镜和RNA测序来分析NP吸收和分子反应.
主要成果:
- 50nm和200nm的PS-NP被累积细胞内化;只有50nm的NP进入了卵细胞.
- 暴露于50nmPS-NPs (3μg/mL) 延迟了卵细胞核成熟和早期胚胎发育.
- RNA测序显示没有差异表达的基因,但表明对线粒体过程的影响,尽管线粒体活动不受影响.
结论:
- 50nm的PS-NP可以透到透明区域,并对卵细胞成熟产生负面影响.
- 观察到的对卵细胞成熟的影响可能不是来自显著的转录或线粒体功能变化.
- 这项研究强调了与纳米塑料暴露相关的潜在生殖风险.
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