载有二米瑞的TPGS/凝接种β-Cyclodextrin/奇托桑接种β-Cyclodextrin纳米输送系统,用于改善口服吸收
Ling Tang1, Jinqing Chen2, Jiali Luo1
1Key Laboratory of Common Technology of Chinese Medicine Preparations, Guangxi University of Chinese Medicine, Nanning 530000, China.
基托桑植入的β-环氧化和凝植入的β-环氧化纳米粒子 (CCGT-DMY-NP) 显著提高了口服二米瑞 (DMY) 的生物可用性. 这种新的配方提高了DMY的稳定性和吸收性,为功能性食品应用提供了一个有前途的策略.
科学领域:
- 食品科学 食品科学 食品科学
- 纳米技术 纳米技术
- 药理学 药理学是指药理学的学科.
背景情况:
- 二米瑞 (DMY) 是葡萄茶的功能成分,具有各种生物活性.
- 由于DMY的稳定性和生物可用性较差,限制了其在食品中的使用.
- 开发先进的输送系统对于提高DMY疗效至关重要.
研究的目的:
- 准备和表征基于基托桑移植β-环氧化 (CS-CD) 和凝移植β-环氧化 (Gel-CD) 的纳米粒子,载有DMY (CCGT-DMY-NPs).
- 评估CCGT-DMY-NP的体外和体内性能,以提高DMY的口服输送.
主要方法:
- CCGT-DMY-NP的制备和表征,包括粒子大小,多分散率指数,泽塔潜力和封装效率.
- 在体外细胞吸收研究使用共聚焦激光扫描显微镜 (CLSM) 和流细胞计 (FCM).
- 在小鼠体内单向肠 perfusion 和 in vivo 药物动力学研究.
主要成果:
- CCGT-DMY-NPs显示了最佳特征:粒子大小为216.33nm,PDI为0.207,泽塔电位为25.67mV,封装效率为98.21%.
- 与自由的DMY相比,Caco-2细胞的细胞吸收增加,肠道透率 (Papp) 和吸收率 (Ka) 显著增加.
- 药理动力学研究显示,CCGT-DMY-NP的AUC0-t增加了2.20倍,相对生物利用率为219.89%.
结论:
- CCGT-DMY-NP有效地提高了DMY的稳定性和口服生物可用性.
- 增强的吸收是由粘膜粘合,紧密结节调和多个内细胞通路介导的.
- 这种基于纳米技术的方法为DMY在功能性食品中的应用提供了一个有希望的策略.
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