调整大米基因表达通过现场促销器切断使用Prime编辑库
Yuying Li1,2, Birong Xu2, Xuanchang Gao2
1Anhui Province Key Laboratory of Rice Germplasm Innovation and Molecular Improvement, Anhui Academy of Agricultural Sciences, Hefei, P. R. China.
Plant biotechnology journal
|February 7, 2026
概括
以Prime编辑为媒介的Promoter Engineering (PEPE) 精确地修改了没有双链断裂 (DSB) 的植物促进器,从而实现了快速的特征发展. 这个平台解码了用于作物改进的促销者逻辑.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组编辑 基因组编辑
- 农作物科学 农作物科学
背景情况:
- 促进器工程对于优化作物特征至关重要,但受到当前基因组编辑工具的精度和可扩展性的限制.
- 现有的方法通常涉及双链断裂 (DSB),带来风险并限制大规模的促销者操纵.
研究的目的:
- 开发一种用于植物促进剂工程的新,精确和可扩展的基因组编辑平台.
- 研究大米D53促进体的功能模块化,并确定监管要素.
主要方法:
- 开发了Prime编辑介导的Promoter Engineering (PEPE),这是一个无DSB系统,利用双向PAM识别和双pegRNA策略.
- 应用PEPE在米D53促销器中进行工程删除,创建了一个突变库.
- 经过验证的编辑精度和评估的编辑基因的遗传稳定性.
主要成果:
- 通过PEPE,在1.8kb的植物促进器中实现了精确的,有的删除.
- 核心促进体区域的删除抑制了70%-85%的基因表达.
- 远端删除揭示了一个神秘的抑制元件,调节转录2.2倍,并将 cis 调节多样性与表型变异联系起来.
结论:
- PEPE提供了一种精确,可扩展和无DSB的方法,用于在植物中操纵千基基尺度的Cis调节元件.
- 该平台可方便复杂的促进物逻辑的解码,并加快具有增强农学特征的作物开发.
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