从0.9%的无菌盐溶液与PBS作为Tritrichomonas胎儿RT-rtPCR测试的采集介质相比,周期值的非劣
Tyler M Jumper1, Merrilee Thoresen1, E Heath King1
1Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
概括
无菌盐水是PBS的合适替代品,用于运输用于Tritrichomonas胎儿 (T.胎儿) 逆转录实时PCR (RT-rtPCR) 测试的样本. 这一发现支持在T. foetus的诊断中使用盐水,这可能会降低牛群的成本.
科学领域:
- 兽医诊断 兽医诊断 兽医诊断 兽医诊断
- 分子生物学分子生物学
- 寄生虫学的寄生虫学
背景情况:
- 菌胎儿 (T. foetus) 感染导致牛犊作业的大量生殖和经济损失.
- 准确且具有成本效益的诊断方法对于管理T.胎儿疫情至关重要.
- 酸盐缓冲盐水 (PBS) 通常被用作用于T. foetus测试的运输介质.
研究的目的:
- 评估无菌盐水与PBS相比作为T.胎儿逆转录实时PCR (RT-rtPCR) 的传输介质的非劣势.
- 为了确定盐水是否影响循环值 (Ct),通过对T.胎儿样本的RT-rtPCR分析得到的值.
主要方法:
- 采集了前皮洗样本,并用已知度的T. foetus生物 (高,中等,低) 接种了疫苗.
- 样品被运输在PBS或无菌盐水中.
- 进行RT-rtPCR分析,并记录周期值 (Ct).
- 使用线性回归和非劣等性t测试来比较两种传输介质之间的Ct值.
主要成果:
- T. 胎儿度显著影响了平均Ct值 (p < 0.0001).
- 无论是传输介质 (p = 0.67) 还是介质与度 (p = 0.87) 之间的相互作用都没有显著影响Ct值.
- 盐水的正常化平均Ct (22.8) 与PBS (22.6) (p = 0.037) 不相差,其非劣势率为1Ct.
结论:
- 无菌盐水是T.胎儿RT-rtPCR测试中PBS的非劣质输送介质.
- 盐水的使用不太可能对T. foetus的诊断结果产生负面影响.
- 盐水为T. foetus诊断中的样品运输提供了一个潜在的更具成本效益的替代方案.
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