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Updated: Feb 9, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

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Published on: August 14, 2019

从Cudrania tricuspidata获得的flavanone 3-hydroxylase和flavonol合成酶的分子鉴定

Jie Fu¹, Jian Liu¹, Xin-Yan Liu²

¹Key Laboratory of Chemical Biology (Ministry of Education), State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

Plant physiology and biochemistry : PPB

|February 7, 2026

概括

研究人员在Cudrania tricuspidata中鉴定出了关键酶 - - 黄3-基酶 (F3H) 和黄醇合成酶 (FLS). 这一发现促进了对黄类生物合成和植物生物的理解.

科学领域:

植物生物化学和分子生物学
代谢工程是代谢工程.
自然产品化学自然产品化学

背景情况:

Cudrania tricuspidata是一种有价值的丝虫料来源,富含黄类.
黄醇生物合成涉及关键酶黄 3-氧化酶 (F3H) 和黄醇合成酶 (FLS).
以前没有在C. tricuspidata中发现F3H和FLS.

研究的目的:

来自C. tricuspidata的F3H和FLS酶的识别和功能性特征.
阐明这些酶的基质特异性和催化机制.
研究CtrF3H1在植物黄类生物合成中的作用.

主要方法:

来自C. tricuspidata的F3H和FLS基因的鉴定和克隆.
在体外酶分析以确定酶活性和基质特异性.
在Arabidopsis thaliana的体内功能特征,包括局部定向的突变发生.

主要成果:

确定了两个F3H酶 (CtrF3H1,CtrF3H2) 和一个FLS酶 (CtrFLS).
CtrF3H1和CtrF3H2表现出flavanone 3-基酶活性;CtrF3H2显示出更广泛的基质范围.
CtrF3H1显示了对纳灵宁的更高的催化效率,并增强了Arabidopsis中的黄/黄醇含量.
CtrFLS将二黄醇转化为黄醇,在突变物中过度表达时增加凯姆菲罗尔和奎尔丁水平.

结论:

已识别的CtrF3H和CtrFLS酶对于C. tricuspidata的黄醇生物合成至关重要.
功能性表征揭示了不同的基质特异性和催化效率,为酶进化提供了洞察力.
这些发现扩大了对C. tricuspidata和其他植物中黄类多样性和代谢途径的理解.

关键词:

科德拉尼亚三角形形形形弗拉瓦-3-基酶的使用方法这是一种黄醇 (flavonol).黄醇合成酶的使用方法功能性表征的功能性表征

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