研究笔记:在 brojler中对Campylobacter jejuni殖民的时间和组织特异性免疫反应
Xin Ye1, Hailey Fugate2, Chrysta N Beck1
1Department of Poultry Science, Mississippi State University, Mississippi State, MS, 39762, USA.
肉中高坎皮洛巴克特 (Campylobacter jejuni) 殖民性与囊中的免疫反应延迟有关,特别是B细胞活动. 这些发现表明,bursal B细胞通路是未来肉干预的关键标记.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 禽畜科学 禽畜科学 禽畜科学
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是一种主要的食源性病原体.
- 在 brojlers 中的 cecal 殖民化对尸体污染作出了重大贡献.
- 了解宿主免疫反应对于缓解C. jejuni持久性至关重要.
研究的目的:
- 研究C. jejuni殖民水平与 brojler免疫组织中的基因表达之间的关系.
- 为了识别C. jejuni在 brojlers中的持久性潜在的免疫标记.
- 为指导减少家禽中C. jejuni的战略.
主要方法:
- 使用不同的C. jejuni剂量 (104和106 CFU/mL) 的小挑战模型.
- 在注射后7天和14天 (DPI) 测量和肠道C. jejuni负载.
- 在Fabricius的脏和囊中对10基因细胞因子组的RT-qPCR分析.
主要成果:
- 在高剂量组中,Cecal C. jejuni的殖民化显著增加了14 DPI.
- 骨基因表达主要受到注射后时间的影响.
- 布尔萨尔基因表达显示出显著的群体和时间相互作用,高剂量组的炎症和B细胞标志物增加了14 DPI.
结论:
- 持续的高CecalC. jejuni殖民与延迟的B细胞中心免疫反应有关.
- 脏免疫反应主要取决于时间,而不是剂量.
- 在14 DPI的B细胞相关途径代表了 brojler干预研究的有希望的终点.
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