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从固定在甲中的样本中,对尿路癌和良性膀组织进行微生物分析

Matt Pearson¹, Phillip A Engen², Stefan J Green³

¹Department of Urology, Rush University Medical Center, Chicago, IL.

Urologic oncology

|February 7, 2026

概括

膀癌 (脑皮细胞癌) 患者与良性疾病相比,具有不同的微生物组. 菌卡尔梅特-盖林 (BCG) 治疗显著改变了膀微生物群,减少了多样性和改变了组成.

科学领域:

微生物组研究的研究.
泌尿器科泌尿器科泌尿器科泌尿器科
在瘤学瘤学.

背景情况:

与宿主相关的微生物群影响免疫和瘤反应.
膀微生物组在尿路细胞癌 (UCC) 中的作用正在出现,但研究不足,特别是在免疫反应和治疗效果方面.
之前的研究主要使用尿样,这可能不完全代表膀环境.

研究的目的:

用FFPE TURBT样本研究尿道细胞癌 (UCC) 患者的膀微生物组合与良性疾病.
评估菌卡尔梅特-盖林 (BCG) 治疗对膀微生物群的影响.

主要方法:

从UCC和良性病理学的膀瘤 (TURBT) 样本中进行甲固定嵌入式 (FFPE) 顺尿道切除的分析.
包括BCG治疗前后患者的样本.
在RNA提取和反转录后,通过16SrRNA安普利康序列的微生物社区特征化.

主要成果:

与良性病理相比,UCC患者表现出更大的微生物多样性和独特的膀微生物组组成.
Streptococcus,Staphylococcus,Actinomyces和Cutibacterium等特定属在良性病例中更为丰富.
在T1-UCC患者中,炎症性属 (例如,Escherichia-Shigella) 和异菌性属 (例如,Enhydrobacter,Micrococcus) 的丰度较高.
在接受BCG治疗的6名患者中,有5名患者表现出微生物多样性的减少和微生物组合的改变, Streptococcus和Rothia的减少.

结论:

从FFPE TURBT标本进行微生物组测序是微生物群评估的可行方法,可以区分UCC和良性病理.
BCG治疗导致膀微生物群的显著枯竭和改变.

关键词:

16S rRNA基因安普利康序列的测序在BCG治疗中,BCG疗法是最有效的.膀中的微生物群.固定在形式上的标本.尿路细胞癌是什么意思

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