开发一种无活化的H9N2亚型禽流感血清DIVA疫苗,采用仿真A/B NA表位体方法
Jingfeng Zhang1, Zhiyu Fan2, Qunxing Pan2
1Key Laboratory of Veterinary Biological Engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China; Zhejiang Difference Biotechnology Co., Ltd., Hangzhou, Zhejiang, PR China; Hefei Baiyu Biotechnology Co., Ltd., Hefei, Anhui, PR China.
开发了一种新的H9N2禽流感DIVA疫苗 (区分感染和接种疫苗的动物). 这种安全有效的标志性疫苗有助于监测,通过区分疫苗接种的鸟类和感染的鸟类,这对H9N2控制至关重要.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 疫苗开发 疫苗开发
背景情况:
- H9N2禽流感对家禽健康和公共安全构成重大威胁.
- 目前的疫苗使疾病监测复杂化,因为它们无法区分受感染和接种疫苗的动物.
研究的目的:
- 开发一种新型的H9亚型区分感染和接种疫苗的动物 (DIVA) 疫苗,用于H9N2禽流感.
- 创建一个标志性疫苗,使得自然感染和接种疫苗的家禽之间的血清学区分.
主要方法:
- 反向遗传学被用来设计一种仿真病毒 (Re-H9-DIVA-J2),通过将神经氨基酶 (NA) 基因替换为来自B/Yamagata血统流感病毒的基因.
- 评估了复合病毒的致病性,生长和遗传稳定性.
- 在无特定病原体 (SPF) 中测试了无活化疫苗的免疫原性和对H9N2病毒的保护功效.
- 进行了血清分析 (IFA,ELISA) 来确认DIVA的兼容性.
主要成果:
- 该Re-H9-DIVA-J2病毒显示出低致病性和B型NA标志物的稳定遗传性.
- 接种了SPF疫苗的产生了强大的抗体标位,并实现了完全的临床保护,病毒分泌量减少了90%以上.
- 接种疫苗的的血清与A型N2蛋白没有交叉反应,使其与感染的动物有区别.
结论:
- 已经开发出一种安全,免疫性和有效的H9标志物疫苗,与DIVA诊断相兼容.
- 这种新型疫苗是增强H9N2禽流感控制和根除战略的有希望的工具.
- 该疫苗有助于改善家禽种群的疾病监测和管理.
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