通过使用多孔石墨碳液态色谱-轨道轨道平行二联式质谱法对异-寡糖化合物进行结合解析分析
Chih-Chieh Lin1, Meng-Jui Lin2, Ju-Hua Chou2
1Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
鉴定异多寡糖 (IMO) 的特征揭示了链接和链条长度对消化能力的影响. 目前的标签可能不准确地反映IMO的成分和血糖影响,需要改进分析框架.
科学领域:
- 碳水化合物化学 碳水化合物化学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 异构-寡糖 (IMO) 的消化能力和血糖反应取决于糖链和链长度.
- 目前用于详细的IMO概况分析的分析方法,特别是超出四糖类的分析方法是有限的.
研究的目的:
- 描述商业产品中40个从二糖到六糖的异-寡糖结构的链接概况.
- 评估商业IMO产品与欧洲食品安全局 (EFSA) 的标准相比.
- 为突出IMO需要先进的标签框架.
主要方法:
- 使用有孔的石墨碳液态色谱与Orbitrap双重质谱法 (PGC-LC-MS/MS) 相结合.
- 分析涵盖了八种商业IMO材料,描述了从分糖到六糖的结构.
主要成果:
- 链接概况主要由α-1→6和α-1→4链接占据主导地位,α-1→4从±10%的二糖体增加到±80%的六糖体.
- 没有商业产品达到EFSA对异盐酸加三糖类-非糖类含量≥90%的干基值.
- 即使标有≥90%IMO标签的产品也只含有~50%α-1→6链接.
结论:
- 商业化的异马尔托-寡糖化合物产品表现出不同于监管预期的可变链接组成.
- 基于总含量的现有标签可能不能充分反映实际的IMO结构及其生理影响.
- 开发链接解决的分析和标签标准对于准确评估IMO消化能力和血糖反应至关重要.
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