用超声波辅助的酶性水解处理的卡梅琳蛋白的结构和功能性质的改变
Ting Li1, Chuping Zhang1, Jianfen Ye2
1State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Food chemistry
|February 7, 2026
概括
超声辅助酶治疗 (UAET) 增强了卡梅林蛋白 (CP) 的功能性质. 这一过程提高了溶解度,结构灵活性和抗氧化能力,导致更好的泡和乳化能力.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物技术是生物技术.
背景情况:
- 卡梅琳蛋白 (CP) 是一种有价值的植物性蛋白质来源.
- 提高CP的功能性质对于其在食品系统中的更广泛应用至关重要.
- 现有的蛋白质修饰方法通常需要恶劣的条件或效率较低.
研究的目的:
- 为了增强卡梅琳蛋白 (CP) 的功能性质.
- 研究超声波辅助酶治疗 (UAET) 对CP的影响.
- 为了将结构变化与功能改进相关联.
主要方法:
- 卡梅琳蛋白接受了超声波处理,随后进行了酶性水解.
- 分析了溶解度,分子量 (SDS-PAGE),结构灵活性 (随机线圈百分比) 和形态.
- 评估了发泡能力,乳化能力和抗氧化能力.
主要成果:
- UAET显著提高了CP溶解度和结构灵活性,增加了随机线圈含量.
- 长达120分钟的水解导致分子量下降,形成了类似网络的结构.
- 在120分钟的水解过程中,发泡能力增加了22%,乳化能力增加了9%.
- 由于释放低分子量,抗氧化能力得到改善.
结论:
- UAET是一种有效的方法,可以改善卡梅林蛋白的功能.
- 观察到的改善与提高溶解度,结构修改和释放有关.
- 修改后的CP显示出需要改进乳化,发泡和抗氧化活性的应用的潜力.
相关概念视频
Structural Protein Function
30.0K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
30.0K
Structural Protein Function
3.3K
3.3K
Mechanical Protein Functions
5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.7K
Hydrolysis of ATP
81.6K
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
81.6K
Protein and Protein Structure
88.2K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
88.2K
Hydrolysis
122.7K
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
122.7K


