对阿格拉稀释和Etest对微稀释进行比较评估,用于测试甲基西林耐药黄金葡萄球菌中对塞夫托比罗尔的敏感性:中国的一项多中心研究
Feiteng Zhu1, Zubi Liu2, Yeqiong Liu1
1Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Journal of global antimicrobial resistance
|February 7, 2026
概括
使用Etest和甲稀释剂对甲素耐药黄金葡萄球菌 (MRSA) 的ceftobiprole敏感性测试显示出高错误率. 需要优化方法来准确检测耐药性和临床使用.
科学领域:
- 微生物学 微生物学
- 临床药房 临床药房
- 传染性疾病 传染性疾病
背景情况:
- 塞夫托比普罗尔是第五代塞法洛斯波林,有效对抗复杂的金黄色葡萄球菌病,包括MRSA.
- 准确的敏感性测试对于指导抗微生物治疗至关重要.
研究的目的:
- 为了比较汁微稀释 (BMD),粉稀释 (AD) 和Etest的性能,以确定对MRSA分离物相比的托比罗尔最小抑制度 (MIC).
- 评估不同MRSA敏感性测试方法之间的一致性和错误率.
主要方法:
- 测试了来自中国多中心研究的60个MRSA分离物.
- 在实验室中,使用AD和Etest评估了 ceftobiprole的活性,其中BMD作为参考方法.
主要成果:
- 骨质检测表明,85%的MRSA分离物对托比醇敏感 (MIC90 = 4 mg/L).
- 无论是AD还是Etest都表现出与BMD的分类和基本一致性不足.
- 对于Etest (22.2%) 和AD (25.0%),特别是ST5和ST239MRSA系,观察到非常严重错误 (VMEs) 的高率.
结论:
- MRSA分离物经常属于CEFTOBIPROL (MIC=2 mg/L) 的EUCAST技术不确定性区域.
- AD和Etest的方法限制导致了错误分类和对抗性的潜在低估.
- 优化敏感性测试策略和精细的解释性指导是必要的,可靠的临床应用ceftobiprole.
相关概念视频
Solution Concentration and Dilution
134.8K
The relative amount of a given solution component is known as its concentration. Often, though not always, a solution contains one component with a concentration that is significantly greater than that of all other components. This component is called the solvent and may be viewed as the medium in which the other components are dispersed or dissolved. Solutions in which water is the solvent are, of course, very common on our planet. A solution in which water is the solvent is called an aqueous...
134.8K
Formation of Dilute Urine
3.3K
The formation of dilute urine is a critical renal adaptation that maintains fluid balance, particularly during periods of high fluid intake. This process primarily involves the juxtamedullary nephrons. By adjusting the permeability of water and ions in response to physiological conditions, the kidneys can either conserve or excrete water, resulting in concentrated or dilute urine.
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...
3.3K
Magnetic Susceptibility and Permeability
2.4K
In linear magnetic materials, like paramagnets and diamagnets, magnetization is proportional to the magnetic field intensity. The constant of proportionality, a dimensionless number, is called magnetic susceptibility. The value of the susceptibility depends on the type of material.
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
2.4K
Serum Studies: Renal Function Tests
448
Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
448
Resistivity
4.6K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K
Resistance
6.0K
When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K


