综合多重PCR和元转录组学揭示了儿科呼吸道感染中上下呼吸道微生物景观
Peilan Wei1, Lu Zhang2, Qingtao Hu3
1Pediatric Pulmonary Department, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Joint International Research Laboratory of Respiratory Health, Guangdong Basic Research Center of Excellence for Respiratory Medicine, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510182, China; China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory, Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangdong Provincial Key Laboratory of Respiratory Disease Research, Guangzhou Medical University; Guangzhou National Laboratory, Bio-Island, Guangzhou 510000, China.
许多儿童呼吸道感染仍然未被诊断出来. 这项研究发现,PCR阴性病例显示出明显的细菌丰富的呼吸道微生物群,因样本地点和疾病严重程度而异,有助于诊断.
科学领域:
- 微生物学 微生物学
- 儿童传染病 儿童传染病
- 基因组学就是基因组学.
背景情况:
- 尽管使用多重PCR,但大量的儿科急性呼吸道感染 (ARTIs) 被归类为未知病因.
- 这些未知病因的ARTIs的微生物特征和临床相关性尚不清楚.
研究的目的:
- 为了比较PCR阳性和PCR阴性儿科ARTIs之间的气道微生物群.
- 研究在ARTIs中的微生物形状,采样地点和疾病严重程度之间的关系.
- 描述小儿ARTIs中的活跃微生物群落和抗生素耐药基因.
主要方法:
- 从514名接受ARTIS治疗的住院儿童中收集了鼻口腔抽样 (NS) 和支气管泡洗液 (BALF).
- 使用22个目标多重PCR面板进行初始病原体识别.
- 在由病原体状态分层的样本上进行了聚合的元转录基因测序,以分析微生物活性和抗生素耐药性基因.
主要成果:
- 多重PCR在77.0%的NS和54.1%的BALF样本中确定了病原体.
- 负PCR样本显示病毒活性较低,细菌转录丰富度较高,具有丰富的Pseudomonas.
- 微生物特征因呼吸道部位和疾病严重程度而异;严重的病例具有更高的细菌负担和 Pseudomonas,而轻微的病例显示出更强烈的病毒信号.
- 在患者样本中检测到抗生素耐药性基因,但在健康对照中没有检测到.
结论:
- PCR阴性儿科ARTIs表现出独特的,细菌丰富的微生物特征.
- 将元转录组学与PCR集成,可以改善ARTIs中的病原体表征.
- 与采样地点和疾病严重程度相关的已识别的微生物模式可以帮助诊断评估和临床管理.
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