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通过CRISPR/Cas9工程的沙门氏菌显示抗菌LL37,增强抗菌活性

Su Jin Jo¹, Se Chang Park¹, Sang Guen Kim²

¹Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.

International journal of antimicrobial agents

|February 7, 2026

概括

具有LL37的转基因菌体对抗抗生素耐药的沙门氏菌 (Salmonella Typhimurium) 具有增强的抗菌活性. 这种菌体治疗方法为抗生素提供了一个有希望的替代品,在临床前模型中证明了安全性和有效性.

科学领域:

微生物学和病毒学
细菌治疗疗法是一种细菌治疗.
抗微生物是一种抗菌.

背景情况:

沙门氏菌 (Typhimurium) 抗生素耐药性的增加需要新的治疗策略.
菌体 (菌体) 为抗生素提供了一个潜在的替代品.
用抗微生物来工程菌体可以提高它们的有效性.

研究的目的:

为了评估一种基因工程细菌的抗菌疗效,该细菌显示抗微生物LL37对沙门氏菌Typhimurium.
评估工程菌体的稳定性,吸附性和溶解活性.
在细胞和体内模型中研究工程菌体的表现.

主要方法:

细菌体的基因工程将LL37显示在病毒结构上.
评估热和pH稳定性,吸附率和细胞溶解活性.
在体外测试使用上皮细胞和体内感染模型 (Galleria mellonella).

主要成果:

工程菌体保持了与野生类型菌体相似的稳定性,但显示了增加的吸附率和增强的抗菌活性.
工程菌体阻止了24-72小时的细菌再生,并显著减少了细菌的附着,内化和细胞内生存.
在体内测试表明Galleria mellonella的剂量依赖性保护,工程菌提供明显更高的疗效比野生类型菌没有可观察到的细胞毒性.

结论:

通过显示LL37等抗微生物来进行菌体工程是一种可行的策略,可以增强抗菌对抗耐药病原体的有效性.
工程菌体在对抗沙门氏菌Typhimurium感染方面表现优于野生类型菌体.
这种方法在治疗抗生素耐药性细菌感染方面具有显著的治疗潜力.

关键词:

抗微生物是一种抗微生物.细菌菌体是一种细菌.菌体工程菌体工程菌体疗法是一种菌体疗法.沙门氏菌 Typhimurium 是一个类型.

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