相关实验视频
Updated: Feb 9, 2026

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Murine Model of Epicutaneously-Induced Immunomodulation
Published on: June 24, 2025
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转录基因分析显示,T细胞介导的Glutamine免疫调节通过CD8A / LCK / LAT信号传递在小猪的肝脏模型中
Guowei Liu1, Yujie Cheng1, Yiyu Zhang1
1Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China; Institute of Animal Nutrition and Feed Science, Guizhou University, Guiyang 550025, China.
The Journal of nutritional biochemistry
|February 7, 2026
概括
谷氨酸 (Gln) 补充剂可以增强小猪肝脏的发育和免疫功能. 它激活了CD8A/LCK/LAT信号轴,突出了Gln在肝脏T细胞信号传递中的免疫调节作用.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 谷氨酸 (Gln) 是一种重要的氨基酸,对免疫反应至关重要.
- 肝脏是新陈代谢和免疫系统的中心器官,在维持健康方面发挥着关键作用.
- 了解 Gln 在肝功能中的分子机制对于其应用至关重要.
研究的目的:
- 研究谷氨酸 (Gln) 调节小猪肝脏功能的分子机制.
- 为了确定饮食中GLN补充对肝脏发育和免疫路径的影响.
主要方法:
- 在断奶小猪中,28天服用1% Gln 的食补充剂.
- mRNA测序以识别差异表达基因 (DEG).
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 蛋白与蛋白相互作用 (PPI) 网络分析.
- 实时定量PCR (RT-qPCR) 和西斑分析.
主要成果:
- Gln补充剂增强了肝脏的发育.
- 确定了444个DEG,其中最丰富的GO术语与免疫系统和免疫反应有关.
- 凯格分析显示T细胞相关免疫通路 (Th1/Th2,Th17分化,T细胞受体信号传递) 的丰富.
- CD8A,LCK和LAT被确定为免疫调节网络中的关键枢纽基因.
- LCK和LAT的蛋白质表达显著增加,证实了CD8A/LCK/LAT信号轴的激活.
结论:
- 谷氨酸 (Gln) 通过影响肝脏T细胞信号传递,在肝脏中表现出免疫调节作用.
- 该研究阐明了Gln在通过CD8A/LCK/LAT通路增强肝脏发育和免疫功能方面的作用.
- 这些发现为进一步研究 Gln 对肝脏的生理影响提供了基础.
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