对比分析揭示了太平洋白Litopenaeus vannamei在不同的病原体相关分子模式刺激后的多样化的免疫反应
Yiming Xu1, Rong Fan1, Peiyu Yan1
1MOE Key Laboratory of Marine Genetics and Breeding (Shandong Key Laboratory of Marine Seed Industry), College of Marine Life Science, Ocean University of China, Qingdao 266003, China; Hainan Key Laboratory of Tropical Aquatic Germplasm (Hainan Seed Industry Laboratory), Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China.
Fish & shellfish immunology
|February 7, 2026
概括
脂聚糖 (LPS) 和糖 (PGN) 都会损害养殖的免疫系统. LPS会导致更严重的损伤和氧化应激,而PGN提供更平衡的免疫反应和组织保护.
科学领域:
- 水产养殖免疫学 水产养殖免疫学
- 海洋生物学 海洋生物学
- 无脊椎动物病理学 无脊椎动物病理学
背景情况:
- 细菌性疾病对养殖的Litopenaeus vannamei构成重大经济威胁.
- 了解对病原体相关分子模式 (PAMPs) 的免疫反应对于疾病控制至关重要.
研究的目的:
- 调查Litopenaeus vannamei对脂多糖 (LPS) 和糖 (PGN) 的独特免疫反应.
- 为了比较由此产生的组织损伤,免疫酶活动和基因表达特征.
主要方法:
- 在Litopenaeus vannamei中注射了LPS,PGN或PBS (对照组).
- 对肝胰腺,中肠,肌肉和进行了组织病理学分析.
- 测量了肝胰腺免疫酶 (AKP,LZM,T-AOC,SOD) 的活性.
- 已识别出差异表达基因 (DEGs) 的肝胰腺的转录基因分析.
主要成果:
- 无论是LPS还是PGN都会导致组织损伤,而LPS会诱导肝大脑中更严重的病变.
- LPS显著增加了AKP和LZM的活动,并减少了T-AOC.
- PGN显著增加了SOD活动.
- LPS诱导了1,253个DEGs,主要与先天免疫有关.
- PGN诱导了182个DEGs,主要参与新陈代谢和细胞修复.
结论:
- LPS强烈激活先天免疫和氧化应激,导致Litopenaeus vannamei的显著组织损伤.
- PGN引发了更保守的免疫调节,平衡了免疫反应和组织保护,导致整体伤害较少.
- 这些发现为针对养殖的细菌疾病的有针对性的策略提供了见解.
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