循环的染色体外圆形DNA作为结直肠癌的预后生物标志物
Xuanmei Luo1,2, Jian Cui3, Jinxin Shi3
1Clinical Biobank, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, No.1 Dahua Road, Dongcheng District, Beijing, 100730, China.
Cell communication and signaling : CCS
|February 7, 2026
概括
血中的异染色体圆形DNA (eccDNA) 显示为早期结直肠癌 (CRC) 复发检测的非侵入性生物标志物具有前途. 这些发现可以改善CRC患者的风险分层和个性化治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 结直肠癌 (CRC) 复发的延迟检测是导致死亡的主要原因.
- 为了有效的CRC管理,需要强大的预后生物标志物.
- 染色体外圆形DNA (eccDNA) 在CRC中的预后作用在很大程度上尚未被探索.
研究的目的:
- 研究血eccDNA特征的预后效用,用于预测CRC复发和死亡率.
- 为了确定与CRC复发相关的特定eccDNA配置文件.
- 评估eccDNA作为CRC预后的非侵入性生物标志物的潜力.
主要方法:
- 一项为期6年的前性队列研究,涉及153个血样本.
- 使用纳米孔测序进行全长eccDNA分析.
- 基于差异性eccDNA签名的复发和死亡率预测模型的构建.
主要成果:
- 复发的患者表现出明显的eccDNA档案,包括来自9号染色体的丰富和更短的中位长度.
- 发起人衍生的eccDNAs,特别是eccPromoter-CARD9,在复发病例中被丰富,并促进了CRC细胞的增殖.
- 含有hsa-mir-374c集群的血eccDNA显示了CRC的高诊断准确性.
- 基于eccDNA的分类器在预测复发 (AUC > 0.8) 和死亡率 (AUC ≥ 0.93) 中取得了高准确性.
结论:
- 血eccDNA景观作为早期CRC预后的强大,非侵入性生物标志物.
- eccDNA分析可以优化CRC患者的风险分层.
- 这些发现支持使用eccDNA指导CRC个性化治疗策略.
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