基于CSSL的GWAS识别了与棉花中Verticillium枯耐药性相关的SNP和候选基因
Youzhong Li1,2, Xiaohui Jiang3, Hongyu Cao1
1Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, 832000, Xinjiang, People's Republic of China.
概括
开发抗病棉花对于作物保护至关重要. 这项研究通过分析染色体段替代线来确定棉花中的新型Verticillium wilt (VW) 耐药基因,提供新的分子育种策略.
科学领域:
- 植物病理学 植物病理学
- 遗传学 是一个遗传学.
- 农业科学 农业科学
背景情况:
- Verticillium wilt (VW) 是全球棉花生产的主要威胁,由土壤传播的真菌Verticillium dahliae引起.
- 繁殖耐高地棉花 (Gossypium hirsutum) 是一个挑战,因为有限的耐药性基因.
- 从海岛棉花 (Gossypium barbadense) 通过跨种类染色体段替代线 (CSSL) 侵入耐药性是一种有前途的策略.
研究的目的:
- 在高地棉花中绘制Verticillium枯耐性的定量特征位置 (QTL).
- 从海岛棉花中识别新的耐药基因和等位基因,用于培育耐药品种.
- 提供候选基因和分子标记物,以增强棉花疾病抵抗力.
主要方法:
- 在三年的实地试验中,对来自Gossypium hirsutum x Gossypium barbadense的318种CSSL进行了表型评估.
- 全基因组关联研究 (GWAS) 使用 130 万个单核酸多态 (SNP).
- 病毒诱导的基因沉默 (VIGS) 以验证候选耐药基因的功能.
主要成果:
- 确定了77个VW电阻QTL,只有12个重叠已知的位置,表明发现了新的电阻区域.
- 在染色体A01和D12上的QTLs内检测到与生物应激反应相关的基因的非同义突变.
- 证明了下调Ghi_D12G018010 (GhHIR1) 降低了Verticillium枯的抵抗力,证实了它的积极作用.
结论:
- CSSL群体是发现棉花中新的Verticillium枯抗性等位基因的宝贵资源.
- GhHIR1是一种候选基因,对棉花中Verticillium枯耐药性作出积极贡献.
- 已识别的QTL和候选基因为标记器辅助选择和分子育种提供了目标,以开发耐药棉花品种.
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