基于建模预测的HPLC-MS/MS与稳定同位素标记:在肝细胞癌中对脂变化的静态动态分析的策略
Zhengkun Tang1, Qingzhe Han1, Yue Chen1
1National and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Journal of chromatography. A
|February 8, 2026
概括
这项研究揭示了葡萄糖和棕酸如何影响肝细胞中的脂,这对于理解肝细胞癌 (HCC) 脂质代谢至关重要. 新的方法可以精确检测和分析这些复杂的分子.
科学领域:
- 生物化学 生物化学
- 代谢学 代谢学 代谢学
- 癌症研究 癌症研究
背景情况:
- 肝细胞癌 (HCC) 与异常的脂代谢有关.
- 由于结构复杂,研究复杂的脂质代谢具有挑战性.
- 稳定同位素标记是代谢研究的一个关键工具.
研究的目的:
- 开发和应用全新方法来全面检测和量化脂.
- 为了研究葡萄糖和棕酸对肝细胞中脂水平的影响.
- 在肝细胞癌的背景下特征化脂变化.
主要方法:
- 结合稳定同位素标记与针对性检测的"建模预测"策略.
- 采用"静态动态"分析方法来研究脂代谢.
- 利用HepG2和LO2细胞系来比较代谢反应.
主要成果:
- 检测到220个脂体,在对葡萄糖,棕酸及其组合的反应中观察到显著的差异表达.
- 在所有测试条件下确定了35种常见的差异表达脂.
- 稳定同位素标签表示葡萄糖比棕酸更广泛地对脂碳池作出贡献.
结论:
- 这项研究为复杂的脂代谢网络提供了新的见解.
- 有关肝细胞癌的特征化脂蛋白变异概况.
- 能够预测脂代谢物合成和消费趋势.
相关概念视频
Isotopes
64.9K
Elements have a set number of protons that determines their atomic number (Z). For example, all atoms with eight protons are oxygen; however, the number of neutrons can vary for atoms of the same element. The sum of the number of protons and the number of neutrons is the mass number (A). Atoms with the same atomic number but different mass numbers are called isotopes. Elements can have multiple isotopes, for example, carbon-12, carbon-13, and carbon-14.
An element's atomic mass, or weight,...
An element's atomic mass, or weight,...
64.9K
Static, Stagnation, Dynamic and Total Pressure
1.5K
The concept of static, stagnation, dynamic, and total pressure is fundamental in fluid dynamics, often explained using Bernoulli's equation:
1.5K
Elements: Chemical Symbols and Isotopes
126.7K
A chemical symbol is an abbreviation used to indicate an element or an atom of an element. For example, the symbol for mercury is Hg. The same symbol is used to indicate one atom of mercury (microscopic domain) or to label a container of many atoms of the element mercury (macroscopic domain).
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
126.7K
Static Equilibrium - I
18.9K
A rigid body is said to be in dynamic equilibrium when both its linear and angular acceleration are zero, relative to an inertial frame of reference. This means that a body in equilibrium can be moving, but only when its linear and angular velocities are constant. A rigid body is said to be in static equilibrium when it is at rest in the selected frame of reference. The distinction between static equilibrium (e.g., a state of rest) and dynamic equilibrium (e.g, a state of uniform motion) is...
18.9K
Static Equilibrium - II
10.0K
Static equilibrium is a special case in mechanics that is very important in everyday life. It occurs when the net force and the net torque on an object or system are both zero. This means that both the linear and angular accelerations are zero. Thus, the object is at rest, or its center of mass is moving at a constant velocity. However, this does not mean that no forces are acting on the object within the system. In fact, there are very few scenarios on Earth in which no forces are acting upon...
10.0K
Static Friction
1.4K
Static friction is a force that opposes the relative motion or tendency of motion between two surfaces in contact. It plays a crucial role in our daily lives, from walking on the ground to driving a car.
For example, consider a scenario where a truck is connected to a car by a rope, ready to tow it along a road. When no external force is applied by the truck, the car remains stationary and is said to be in static equilibrium. In this case, the forces acting on the car, such as gravity and the...
For example, consider a scenario where a truck is connected to a car by a rope, ready to tow it along a road. When no external force is applied by the truck, the car remains stationary and is said to be in static equilibrium. In this case, the forces acting on the car, such as gravity and the...
1.4K


