在各种眼部感染中进行基因组测序:对诊断有用性的系统性审查
Made Susiyanti1, Florentina Febrina2, Ikhwanuliman Putera3
1Department of Ophthalmology, Faculty of Medicine University of Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Survey of ophthalmology
|February 8, 2026
概括
大基因组下一代测序 (mNGS) 显示出在眼部感染中识别多种病原体的前景. 这种无偏见的方法有助于检测难以找到的生物和评估抗菌素耐药性,改善诊断.
科学领域:
- 眼科医生 眼科 眼科
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 在全球范围内,眼部感染导致明显的视力损伤.
- 眼部感染的常规诊断方法存在挑战.
- 甲基因组下一代测序 (mNGS) 为病原体检测提供了一个公正的方法.
研究的目的:
- 评估mNGS在眼部感染中的诊断性能.
- 突出mNGS在临床应用中的优势和局限性.
- 在日常眼科实践中探索mNGS的未来方向.
主要方法:
- 21项研究的系统审查和元分析.
- 在各种眼部感染病例中包括1219只眼睛.
- 对mNGS阳性率,敏感性和特异性的分析.
主要成果:
- mNGS检测到广泛的病原体,包括病毒,细菌,真菌和寄生虫.
- 病原体检测发生在属和物种层面.
- mNGS确定了与抗菌素耐药性 (AMR) 相关的基因和突变.
结论:
- mNGS是眼部感染病原体识别的宝贵工具,特别是难以捉摸的生物.
- 对于mNGS结果的临床实用性,需要仔细解释.
- 需要使用标准化协议进行进一步的大规模研究,以便在临床实践中优化mNGS.
相关概念视频
Review and Preview
8.4K
In statistics, several tools are used to interpret the data. Measures of central tendency represent the characteristics of the data, such as mean, median, and mode. Additionally, measures of variance like standard deviation and range are used to find the spread of data from the mean. Relative standing measures the distance between data locations. Commonly used measures of relative standings are percentile, z score, and quartiles.
Percentiles are a type of fractile that partition data into...
Percentiles are a type of fractile that partition data into...
8.4K
Review and Preview
11.6K
Data are individual items of information obtained from a population or sample. Data may be classified as qualitative (categorical), quantitative continuous, or quantitative discrete. Because it is not practical to measure the entire population in a study, researchers use samples to represent the population. A random sample is a representative group from the population chosen by using a method that gives each individual in the population an equal chance of being included in the sample. Random...
11.6K
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care
323
A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
323
Random and Systematic Errors
15.2K
Scientists always try their best to record measurements with the utmost accuracy and precision. However, sometimes errors do occur. These errors can be random or systematic. Random errors are observed due to the inconsistency or fluctuation in the measurement process, or variations in the quantity itself that is being measured. Such errors fluctuate from being greater than or less than the true value in repeated measurements. Consider a scientist measuring the length of an earthworm using a...
15.2K
Systematic Sampling Method
13.4K
Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
Systematic sampling is one of the simplest methods...
Systematic sampling is one of the simplest methods...
13.4K
Propagation of Uncertainty from Systematic Error
1.5K
The atomic mass of an element varies due to the relative ratio of its isotopes. A sample's relative proportion of oxygen isotopes influences its average atomic mass. For instance, if we were to measure the atomic mass of oxygen from a sample, the mass would be a weighted average of the isotopic masses of oxygen in that sample. Since a single sample is not likely to perfectly reflect the true atomic mass of oxygen for all the molecules of oxygen on Earth, the mass we obtain from this...
1.5K


