Limosilactobacillus fermentum LF61:从基因组学到临床应用的安全性和功能性的多维研究
Manfei Jin1, Fei Xu2, Yinhua Liu2
1Plateau Biomedical Big Data Mining, and Bioinformatics Analysis Laboratory, Xizang Minzu University, Xianyang, 712082, China; Provincial Key Laboratory of Molecular Genetic Mechanism and Intervention of Diseases related to the Tibetan Plateau, Xizang Minzu University, Xianyang, 712082,China.
概括
Limosilactobacillus fermentum LF61是一种来自人乳的安全益生菌,可以增强肠道免疫力. 临床试验表明它可以增强抗菌和免疫球蛋白,支持其作为免疫调节益生菌的使用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- Limosilactobacillus fermentum LF61是一种来自母乳的菌株.
- 益生菌调节肠道微生物和免疫系统.
- 了解LF61的安全性和有效性对于其应用至关重要.
研究的目的:
- 综合评估 Limosilactobacillus fermentum LF61.1. 的安全性和功能疗效.
- 研究LF61对肠道微生物群和宿主免疫反应的影响.
- 提供LF61作为免疫调节益生菌的证据.
主要方法:
- 对毒性和耐药性基因进行基因组分析.
- 在体外生存测定 (胃/肠道条件) 和细胞毒性测试.
- 在小鼠中进行的急性口服毒性研究 (LD50的确定).
- 随机,双盲临床试验评估免疫标记物 (LL-37,IgA,IgG,IgM).随机,双盲临床试验评估免疫标记物 (LL-37,IgA,IgG,IgM).
- 肠道微生物组组成和功能的元基因组学分析.
主要成果:
- LF61基因组缺乏毒性因子和抗生素耐药性基因,符合安全标准.
- 在模拟的胃肠道条件下高体外生存率,对Caco-2细胞无毒.
- 在小鼠中,急性口服毒性LD50 > 2 × 10^10 CFU/kg.
- 临床试验显示血清LL-37 (12.3%) 和免疫球蛋白 (IgA,IgG,IgM) 的增加.
- LF61促进了有益细菌,激活了碳水化合物代谢,并保持了微生物群的多样性.
结论:
- Limosilactobacillus fermentum LF61是一种安全且功能有效的益生菌.
- 通过双向调节机制,LF61调节肠免疫轴.
- LF61显示出作为一种新的免疫调节益生菌的潜力,用于肠道健康.
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