在多通道微流体芯片上使用单纳米粒子LSPR生物传感器对miRNA-451进行无放大和现场检测系统
Kuilin Liu1, Xingyu Zi1, Jianqi Wang1
1College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China; Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, Tianjin 300350, China.
Analytical biochemistry
|February 8, 2026
概括
这项研究介绍了一种用于早期结直肠癌 (CRC) 检测的无放大传感系统. 它使用局部表面等离子体共振 (LSPR) 和微流体检测生物标记物microRNA-451 (miRNA-451) 的高灵敏度.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 分子诊断学 分子诊断
背景情况:
- 早期对结直肠癌 (CRC) 的查对于降低死亡率至关重要.
- 准确检测像microRNA-451 (miRNA-451) 这样的生物标志物对于及时诊断至关重要.
- 现有的miRNA检测方法可能很复杂,缺乏灵敏度.
研究的目的:
- 开发一种无放大,高灵敏的传感系统,用于在现场检测miRNA-451.1.
- 使用局部表面等离子体共振 (LSPR) 技术与多通道微流体芯片集成.
- 为早期结直肠癌生物标志物检测建立一个强大的平台.
主要方法:
- 开发了一种使用金纳米粒子 (AuNPs) 固定在微流体芯片上的单链DNA (ssDNA) 探针上的传感系统.
- 利用LSPR技术通过与ssDNA探针进行特定杂交来检测miRNA-451,形成AuNPs二元体.
- 采用单个纳米粒子追踪策略来分析光谱红移,量化miRNA-451并减轻采样错误.
主要成果:
- 对于miRNA-451.1,已达到19.2 fM的检测极限 (LOD).
- 用ssDNA探针证明了miRNA-451的特定杂交,在LSPR散射光谱中诱导了可测量的红移.
- 通过使用精确的单纳米粒子追踪策略,成功地减轻了空间采样错误.
结论:
- 开发的系统提供了无放大,高度灵敏的miRNA-451.1的检测.
- 集成的LSPR和微流体平台提供了更高的检测效率和准确性.
- 这项技术作为早期结直肠癌查和诊断的强大平台.
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